Both authors contributed equally to this work.
Expression of HMGA2 in bladder cancer and its association with epithelial-to-mesenchymal transition
Article first published online: 26 FEB 2014
© 2014 John Wiley & Sons Ltd
Volume 47, Issue 2, pages 146–151, April 2014
How to Cite
Ding, X., Wang, Y., Ma, X., Guo, H., Yan, X., Chi, Q., Li, J., Hou, Y. and Wang, C. (2014), Expression of HMGA2 in bladder cancer and its association with epithelial-to-mesenchymal transition. Cell Proliferation, 47: 146–151. doi: 10.1111/cpr.12096
- Issue published online: 12 MAR 2014
- Article first published online: 26 FEB 2014
- Manuscript Accepted: 5 NOV 2013
- Manuscript Received: 22 AUG 2013
High mobility group protein2 (HMGA2) and epithelial-to-mesenchymal transition are both related to progress of bladder cancer, however, the relationship between HMGA2, E-cadherin and vimentin in bladder cancer is not yet known. Thus, this study has examined expression of HMGA2, E-cadherin and vimentin in bladder cancer and investigated their relationship.
Materials and methods
The 5637 bladder cancer cell line and SV-HUC-1 normal uroepithelial cells were used to study expression of HMGA2, E-cadherin and vimentin using RT-PCR and western blotting. Paraffin wax-embedded bladder cancer tissues were used to study protein expression using immunohistochemistry and χ2 analysis and Kendall's correlation were utilized statistical methods.
Overexpression of HMGA2 was associated with down-regulation of E-cadherin and up-regulation of vimentin in the 5637 bladder cancer line. A total of 49 paraffin wax-embedded tissues of transitional cell bladder cancer were used. Positive expression levels of HMGA2 protein and vimentin were 41 and 43% in bladder tissues, respectively. No expression of E-cadherin was found in 43%. Expression of HMGA2, loss of E-cadherin and expression of vimentin are all significantly correlated with bladder cancer grade and stage. Loss of E-cadherin and expression of vimentin both correlated with recurrence of the bladder cancer.
Expression of HMGA2 was closely associated with occurrence of epithelial-to-mesenchymal transition. Expression of HMGA2, loss of E-cadherin and expression of vimentin may indicate high degree malignancy of bladder cancer. Loss of E-cadherin expression and positive expression of vimentin may predict recurrence of bladder cancer.