Live-cell analysis of plant reproduction: Live-cell imaging, optical manipulation, and advanced microscopy technologies

Authors

  • Daisuke Kurihara,

    Corresponding author
    1. JST, ERATO, Higashiyama Live-Holonics Project, Nagoya, Aichi, Japan
    • Division of Biological Science, Graduate School of Science, Nagoya, Aichi, Japan
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  • Yuki Hamamura,

    1. Division of Biological Science, Graduate School of Science, Nagoya, Aichi, Japan
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  • Tetsuya Higashiyama

    Corresponding author
    1. JST, ERATO, Higashiyama Live-Holonics Project, Nagoya, Aichi, Japan
    2. Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Nagoya, Aichi, Japan
    • Division of Biological Science, Graduate School of Science, Nagoya, Aichi, Japan
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Authors to whom all correspondence should be addressed.

Emails: kuri@bio.nagoya-u.ac.jp; higashi@bio.nagoya-u.ac.jp

Abstract

Sexual reproduction ensures propagation of species and enhances genetic diversity within populations. In flowering plants, sexual reproduction requires complicated and multi-step cell-to-cell communications among male and female cells. However, the confined nature of plant reproduction processes, which occur in the female reproductive organs and several cell layers of the pistil, limits our ability to observe these events in vivo. In this review, we discuss recent live-cell imaging in in vitro systems and the optical manipulation techniques that are used to capture the dynamic mechanisms representing molecular and cellular communications in sexual plant reproduction.

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