Practical tips for imaging ascidian embryos

Authors

  • Takefumi Negishi,

    1. UPMC Univ Paris 06 and Centre National de la Recherche Scientifique, Laboratoire de Biologie du Développement de Villefranche-sur-Mer, Observatoire Océanologique, Villefranche-sur-mer, France
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  • Alex McDougall,

    1. UPMC Univ Paris 06 and Centre National de la Recherche Scientifique, Laboratoire de Biologie du Développement de Villefranche-sur-Mer, Observatoire Océanologique, Villefranche-sur-mer, France
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  • Hitoyoshi Yasuo

    Corresponding author
    • UPMC Univ Paris 06 and Centre National de la Recherche Scientifique, Laboratoire de Biologie du Développement de Villefranche-sur-Mer, Observatoire Océanologique, Villefranche-sur-mer, France
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Author to whom all correspondence should be addressed.

Email: yasuo@obs-vlfr.fr

Abstract

Decades of studies on ascidian embryogenesis have culminated in deciphering the first gene regulatory “blueprint” for the generation of all major larval tissue types in chordates. However, the current gene regulatory network (GRN) is not well integrated with the morphogenetic and cellular processes that are also taking place during embryogenesis. Describing these processes represents a major on-going challenge, aided by recent advances in imaging and fluorescent protein (FP) technologies. In this report, we describe the application of these technologies to the developmental biology of ascidians and provide a detailed practical guide on the preparation of ascidian embryos for imaging.

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