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- The principle of SiMS microscopy
- Quantitative modeling of multiple SiMS data on actin and actin-associated proteins to investigate actin treadmilling in lamellipodia
- Comparison between SiMS and FRAP data implies recycling with actin oligomers
- s-FDAPplus elucidates the mechanosensitive G-actin increase that leads to actin nucleation burst of formins
Live-cell single-molecule imaging is a powerful tool to elucidate the in vivo biochemistry of cytoskeletal proteins. However, it is often somewhat difficult to interpret how a bulk population of the observed molecule might behave as a whole. We review our recent studies in which the combination of image analysis with modeling and bulk kinetics measurements such as FRAP (fluorescence recovery after photobleaching) clarified basic problems in the regulation of actin remodeling pathways.