“Brainbow” imaging of developing calyx-type presynaptic terminals in the chick ciliary ganglion. A plasmid vector containing three membrane-tethered fl uorescent proteins, m-CFP, m-YFP and m-RFP, which are flanked by loxP and its variants, were electroporated in the E2 midbrain in ovo with a plasmid containing NCre. See Yawo et al., 474-490.

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