- Top of page
- Zinc-finger nucleases
- Transcription activator-like effector nucleases
- Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein
- Advantages of the site-specific nuclease technologies
The laboratory rat has been widely used as an animal model in biomedical science for more than 150 years. Applying zinc-finger nucleases or transcription activator-like effector nucleases to rat embryos via microinjection is an efficient genome editing tool for generating targeted knockout rats. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonucleases have been used as an effective tool for precise and multiplex genome editing in mice and rats. In this review, the advantages and disadvantages of these site-specific nuclease technologies for genetic analysis and manipulation in rats are discussed.