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Inhibition of Fibroblast Proliferation In Vitro Using Low-Level Infrared Light-Emitting Diodes

Authors

  • Hadar Lev-Tov MD,

    1. Department of Dermatology, University of California at Davis, Sacramento, California
    2. Dermatology Service, Sacramento Veterans Affairs Medical Center, Mather, California
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  • Neil Brody MD, PhD,

    1. Department of Dermatology, State University of New York Downstate, Brooklyn, New York
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  • Daniel Siegel MD, MS,

    1. Department of Dermatology, State University of New York Downstate, Brooklyn, New York
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  • Jared Jagdeo MD, MS

    Corresponding author
    1. Dermatology Service, Sacramento Veterans Affairs Medical Center, Mather, California
    2. Department of Dermatology, State University of New York Downstate, Brooklyn, New York
    • Department of Dermatology, University of California at Davis, Sacramento, California
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  • Photomedex loaned the device used in the paper to the authors. Dr. Siegel is on the Photomedex Scientific Advisory Board.

Address correspondence and reprint requests to: Jared Jagdeo, MD, MS, 3301 C Street, Suite 1400, Sacramento, CA 95816, or e-mail: jrjagdeo@gmail.com

Abstract

Background

Scars, including hypertrophic and keloidal-type scars, may occur after burns, trauma, or surgery. Despite several treatment options available for scars, few effective, noninvasive modalities exist. Recently, a few small clinical studies revealed the possible benefit of red and infrared (IR) low-level light therapy (LLLT) in scar treatment. One of the important features of scars is proliferation of dermal fibroblasts, but in vitro data regarding the effects of light-emitting diode (LED)-generated IR light on human skin fibroblasts is lacking.

Objective

To evaluate the effect of IR LLLT generated using LEDs on fibroblast proliferation and viability in vitro.

Methods and Materials

Irradiation of normal human skin fibroblasts using IR LED panels was performed in vitro, and modulation of proliferation and viability was quantified using Trypan blue dye exclusion assay.

Results

Fluences of 80, 160 and 320 J/cm2 resulted in statistically significantly less fibroblast proliferation than in controls, without statistically significantly less cellular viability.

Conclusion

IR LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars.

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