C. C. Cheng and P. Weerateerangkul contributed equally to this study.
Apelin regulates the electrophysiological characteristics of atrial myocytes
Article first published online: 28 OCT 2012
© 2012 The Authors. European Journal of Clinical Investigation © 2012 Stichting European Society for Clinical Investigation Journal Foundation
European Journal of Clinical Investigation
Volume 43, Issue 1, pages 34–40, January 2013
How to Cite
Cheng, C.-C., Weerateerangkul, P., Lu, Y.-Y., Chen, Y.-C., Lin, Y.-K., Chen, S.-A. and Chen, Y.-J. (2013), Apelin regulates the electrophysiological characteristics of atrial myocytes. European Journal of Clinical Investigation, 43: 34–40. doi: 10.1111/eci.12012
- Issue published online: 20 DEC 2012
- Article first published online: 28 OCT 2012
- Accepted manuscript online: 25 SEP 2012 10:10AM EST
- Received 22 February 2012; accepted 18 September 2012
- cardiac electrophysiology;
- isolated cardiomyocytes
Eur J Clin Invest 2012
Backgroud Apelin, a potential agent for treating heart failure, has various ionic effects on ventricular myocytes. However, the effects of apelin on the atrium are not clear. The purpose of this study was to investigate the acute effects of apelin on the electrophysiological characteristics of atrial myocytes.
Method Whole-cell patch-clamp techniques were used to investigate the action potential (AP) and ionic currents in isolated rabbit left atrial (LA) myocytes before and after the administration of apelin.
Result Apelin reduced LA AP duration measured at 90%, 50% and 20% repolarization of the amplitude by 11 ± 3%, 24 ± 5%, 30 ± 7% at 1 nM (n = 11), and by 14 ± 4%, 36 ± 6% and 45 ± 5% at 10 nM (n = 11), but not at 0·1 nM. Apeline (0·1, 1, 10 nM) did not change the amplitude, or resting membrane potential in LA myocytes. Apelin (1 nM) increased sodium currents, ultra-rapid potassium currents and the reverse mode of sodium-calcium exchanger currents, but decreased late sodium currents and L-type calcium currents and did not change transient outward currents or inward rectifier potassium currents in LA myocytes.
Conclusions Apelin significantly changed the atrial electrophysiology with a shortening of AP duration, which may be caused by its effects on multiple ionic currents.