Prognostic relevance of BRD7 expression in colorectal carcinoma

Authors

  • Wen-Jing Wu,

    1. State Key Laboratory of Oncology in Southern China, Guangzhou, China
    2. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
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    • These authors contributed equally to this work.
  • Kai-Shun Hu,

    1. Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Research Center of Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China
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    • These authors contributed equally to this work.
  • Dong-Liang Chen,

    1. State Key Laboratory of Oncology in Southern China, Guangzhou, China
    2. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
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  • Zhao-Lei Zeng,

    1. State Key Laboratory of Oncology in Southern China, Guangzhou, China
    2. Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, China
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  • Hui-Yan Luo,

    1. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
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  • Feng Wang,

    1. State Key Laboratory of Oncology in Southern China, Guangzhou, China
    2. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
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  • De-Shen Wang,

    1. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
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  • Zhi-Qiang Wang,

    1. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
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  • Fan He,

    1. Department of Forensic Medicine, Sun Yat-sen University, Guangzhou, China
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  • Rui-Hua Xu

    Corresponding author
    1. Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China
    • State Key Laboratory of Oncology in Southern China, Guangzhou, China
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Correspondence to: Rui-hua Xu, Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. Tel.: 86 20 8734 3228; fax: 86 20 8734 3228; e-mail: xurh@sysucc.org.cn

Abstract

Background

BRD7 is a member of bromodomain-containing protein and was found to be a cofactor of P53. Down-regulation of BRD7 has been shown in nasopharyngeal carcinoma cell lines and tissues. However, the clinical role of BRD7 in colorectal cancer remains unknown.

Materials and methods

Real-time PCR, Western blotting analysis and immunohistochemistry were employed to examine BRD7 expression in CRC cell lines/tissues compared with normal epithelia cells/adjacent non-tumorous tissues. In addition, statistical analyses were applied to evaluate the diagnostic value and associations of BRD7 expression with clinical parameters of patient samples.

Results

BRD7 was down-regulated in colorectal cancer cell lines and cancerous tissues compared with that in normal colon epithelial cells and adjacent noncancerous tissue samples. BRD7 protein expression was positively correlated with clinical stage ( <  0·001), T classification (P  =  0·001), N classification ( <  0·001), M classification (P <  0·001) and pathologic differentiation (P =  0·008). Patients with low/none BRD7 expression had shorter overall survival time than those with higher BRD7 expression. Univariate and multivariate analyses indicated BRD7 expression was an independent prognostic factor (< 0·001).

Conclusion

BRD7 may serve as a potential prognostic biomarker of human colorectal cancer.

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