Endothelial function does not relate to haemoglobin or serum erythropoietin concentrations and these do not explain the gender difference in endothelial function in healthy middle-aged men and women
Version of Record online: 18 JAN 2013
© 2012 The Authors. European Journal of Clinical Investigation © 2012 Stichting European Society for Clinical Investigation Journal Foundation
European Journal of Clinical Investigation
Volume 43, Issue 3, pages 225–230, March 2013
How to Cite
Eur J Clin Invest 2013; 43 (3): 225–230
- Issue online: 14 FEB 2013
- Version of Record online: 18 JAN 2013
- Accepted manuscript online: 26 NOV 2012 12:38PM EST
- Manuscript Accepted: 21 NOV 2012
- Manuscript Received: 19 MAY 2012
- Guy's and St Thomas' Charity. Grant Number: R070722
- Food Standards Agency
- Guy's & St Thomas' NHS Foundation Trust
- Cardiovascular risk;
- flow mediated dilation;
- nitric oxide;
Haemoglobin scavenges nitric oxide, and a previous study has shown a negative association between flow-mediated vasodilation (FMD), a measure of nitric oxide (NO)-dependent vasomotor function and haemoglobin concentrations [Hb]. Circulating erythropoietin (EPO) is also negatively associated with [Hb] and could influence availability of NO. The purpose of this study was to examine the association of FMD with [Hb] and EPO concentrations and to determine whether these contribute to the sex difference in FMD. FMD (by high-resolution ultrasound), [Hb], circulating immunoreactive EPO and cardiovascular risk factors were measured in 317 healthy middle-aged men and women (183 women, 33 premenopausal, mean age ± SD, 55 ± 6·8 years) participating in a dietary study.
In the whole mixed-sex group, FMD was negatively correlated with [Hb] (R = −0·23, P < 0·001). However, in a multivariable model, incorporating sex and other confounding factors, FMD was independently negatively correlated only with age, male sex and systolic blood pressure: standardized regression coefficients −0·21 (P < 0·01), −0·17 (P < 0·05) and −0·20 (P < 0·05) respectively and not with [Hb]. Similarly, when the analysis was restricted to men or to postmenopausal women, there was no significant relationship between FMD and Hb. There was no significant correlation between FMD and EPO on either univariate analysis in the whole group, in each sex, or in multivariate analysis.
These results suggest that in healthy middle-aged subjects, FMD is not influenced by [Hb] or EPO and these do not contribute to the gender difference in FMD.