Conflict of interest and disclosures of financial support claims: none
The impact of gender and serum estradiol levels on HDL-mediated reverse cholesterol transport
Article first published online: 9 FEB 2013
© 2012 The Authors. European Journal of Clinical Investigation © 2012 Stichting European Society for Clinical Investigation Journal Foundation
European Journal of Clinical Investigation
Volume 43, Issue 4, pages 317–323, April 2013
How to Cite
Eur J Clin Invest 2013; 43 (4): 317–323
- Issue published online: 18 MAR 2013
- Article first published online: 9 FEB 2013
- Accepted manuscript online: 19 DEC 2012 05:40PM EST
- Manuscript Accepted: 17 DEC 2012
- Manuscript Received: 24 JUL 2012
- Research Council for the Health, Academy of Finland. Grant Number: 132629
- Sigrid Juselius Foundation
- Päivikki ja Sakari Sohlberg Foundation
- Finnish Heart Foundation
- high-density lipoproteins;
- lecithin: cholesterol acyltransferase;
- phospholipid transfer protein;
- reverse cholesterol transport
Premenopausal women have a lower incidence of cardiovascular disease compared to men of the same age. Endogenous oestrogens, especially estradiol, presumably protect against atherosclerosis by a variety of mechanisms. Reverse cholesterol transport (RCT) mechanisms also provide protection against this disease. RCT is defined as the removal of cholesterol from peripheral macrophage foam cells, via high-density lipoproteins (HDL), and cholesterol transportation to the liver for excretion. We have previously shown in a preliminary study that HDL, isolated from premenopausal women, enhanced macrophage cholesterol efflux compared to HDL derived from age-matched male subjects.
Materials and methods
Here, we expanded this study by analysing a larger population of healthy volunteers and evaluated the capacity of HDL derived from women with high or low serum E2 concentrations, mainly representing premenopausal and postmenopausal women, respectively, or men (each group consisting of 30 subjects) to facilitate cholesterol removal from human THP-1 macrophages. HDL isolated from serum samples was incubated with [3H] cholesterol oleate-loaded macrophages for 16 h, after which cholesterol efflux to HDL was determined.
No significant differences in the efflux-promoting ability of HDL existed among the three groups. Relevant plasma factors involved in further steps of RCT, such as cholesterol ester transfer protein (CETP), phospholipid transfer protein (PLTP) and lecithin:cholesterol acyltransferase (LCAT) activities were also analysed, but no differences were observed among the study groups.
The results do not support a role for estradiol status or gender in modifying the initial step of RCT as a protective mechanism against cardiovascular disease.