The evolution and function of the Pax/Six regulatory network in sponges
Article first published online: 23 APR 2013
© 2013 Wiley Periodicals, Inc.
Evolution & Development
Volume 15, Issue 3, pages 186–196, May 2013
How to Cite
Rivera, A., Winters, I., Rued, A., Ding, S., Posfai, D., Cieniewicz, B., Cameron, K., Gentile, L. and Hill, A. (2013), The evolution and function of the Pax/Six regulatory network in sponges. Evolution & Development, 15: 186–196. doi: 10.1111/ede.12032
- Issue published online: 23 APR 2013
- Article first published online: 23 APR 2013
- Jeffress Memorial Trust (A.H.)
- NSF (A.H.)
- Beckman Foundation (I.W.)
- Merck/AAS (I.W., A.R., B.C., S.D.)
- HHMI (D.P.)
- University of Richmond (S.D., A.R.)
Examining the origins of highly conserved gene regulatory networks (GRNs) will inform our understanding of the evolution of animal body plans. Sponges are believed to be the most ancient extant metazoan lineage, and as such, hold clues about the evolution of genetic programs deployed in animal development. We used the emerging freshwater sponge model, Ephydatia muelleri, to study the evolutionary origins of the Pax/Six/Eya/Dac (PSED) GRN. Orthologs to Pax and Six family members are present in E. muelleri and are expressed in endothelial cells lining the canal system as well as cells in the choanoderm. Knockdown of EmPaxB and EmSix1/2 by RNAi resulted in defects to the canal systems. We further show that PaxB may be in a regulatory relationship with Six1/2 in E. muelleri, thus demonstrating that a component of the PSED network was present early in metazoan evolution.