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Additional Supporting Information may be found in the online version of this article at the publisher's web-site.

FilenameFormatSizeDescription
ede12039-sm-0001-SuppFig-S1.tif1199KFig. S1. Predicted secondary structures of P. marinus miR-1 and miR-133 precursors. Pink and orange circles indicate mature regions and seed sequences, respectively.
ede12039-sm-0002-SuppFig-S2.tif1983KFig. S2. Predicted secondary structures of C. milii miR-1 and miR-133 precursors. Pink and orange circles indicate mature regions and seed sequences, respectively.
ede12039-sm-0003-SuppFig-S3.tif557KFig. S3. Genomic sequences surrounding miR-1-3 and miR-133-2 in the chimaera (elephant shark Collorhinchus milii). (A) Diagram showing locations of miR genes in the genomic sequence corresponding to the five mutually exclusive contigs (http://esharkgenome.imcb.a-star.edu.sg). Yellow and pink arrows indicate miR-1 and miR-133 genes, respectively, in the 5′–3′ direction. Distance between the miR genes and lengths of the contigs are not in scale. (B) Genomic DNA sequence surrounding cmi-mir-1-3 and cmi-mir-133-2. Predicted precursor sequences are shaded in yellow and pink. The intervening sequence between the miRs is not shown in full and is represented by dots. Mature miR sequences are double-underlined. Star (*) sequences are single-underlined. Primers used in the genomic PCR are indicated by arrows (see Materials and Methods).

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