Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain

Authors


Summary

A TaqMan real-time PCR assay was developed for Phytophthora austrocedrae, an emerging pathogen causing severe damage to juniper in Britain. The primers amplified DNA of the target pathogen down to 1 pg of extracted DNA, in both the presence and absence of host DNA, but did not amplify any of the non-target Phytophthora and fungal species tested. The assay provides a useful tool for screening juniper populations for the disease.

Ancillary