Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris (pages 2064–2079)
Bassam Al Atalah, Elke Fouquaert, Dieter Vanderschaeghe, Paul Proost, Jan Balzarini, David F. Smith, Pierre Rougé, Yi Lasanajak, Nico Callewaert and Els J. M. Van Damme
Version of Record online: 16 MAY 2011 | DOI: 10.1111/j.1742-4658.2011.08122.x
The Oryza sativa lectin (Orysata) is located in the nucleus and the cytoplasm of the plant cell. This lectin was expressed in the medium of the methylotrophic yeast Pichia pastoris and purified. Glycan array analysis revealed that Orysata interacts with high-mannose and more complex N-glycan structures. Orysata has potent anti-human immunodeficiency virus and anti-respiratory syncytial virus activity in cell culture.