Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase (pages 2469–2484)
Helena Ryšlavá, Alžběta Kalendová, Veronika Doubnerová, Přemysl Skočdopol, Vinay Kumar, Zdeněk Kukačka, Petr Pompach, Ondřej Vaněk, Kristýna Slámová, Pavla Bojarová, Natallia Kulik, Rudiger Ettrich, Vladimír Křen and Karel Bezouška
Article first published online: 1 JUN 2011 | DOI: 10.1111/j.1742-4658.2011.08173.x
Fungal β-N-acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship with other fungal hexosaminidases. Results from molecular dynamics simulations and substrate docking supported the experimental kinetic and substrate specificity data, and identified a secondary binding site for the substrate close to the catalytic site.