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The FEBS Journal

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August 2011

Volume 278, Issue 16

Pages 2753–2962

  1. Review Articles

    1. Top of page
    2. Review Articles
    3. Original Articles
    4. Author Index
    1. You have free access to this content
      Molecular basis of glyphosate resistance – different approaches through protein engineering (pages 2753–2766)

      Loredano Pollegioni, Ernst Schonbrunn and Daniel Siehl

      Version of Record online: 28 JUN 2011 | DOI: 10.1111/j.1742-4658.2011.08214.x

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      Glyphosate is the most-used herbicide in the world. Starting in 1996, transgenic glyphosate-resistant plants were introduced thus allowing to remove emerged weeds without crop damage. This review focuses on the evolution of mechanisms of resistance to glyphosate as obtained through natural diversity, the gene shuffling approach to molecular evolution, and a rational, structure-based approach to protein engineering.

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      Applications and trends in systems biology in biochemistry (pages 2767–2857)

      Katrin Hübner, Sven Sahle and Ursula Kummer

      Version of Record online: 26 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08217.x

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      Systems biology has received ever increasing interest during the last decade. In this review we focus on applications of systems biology approaches that have been employed to shed light on biochemical functions and mechanisms previously unknown. We point out which computational and experimental methods are employed most frequently and which trends in systems biology research can be observed.

  2. Original Articles

    1. Top of page
    2. Review Articles
    3. Original Articles
    4. Author Index
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      The association of heavy and light chain variable domains in antibodies: implications for antigen specificity (pages 2858–2866)

      Anna Chailyan, Paolo Marcatili and Anna Tramontano

      Version of Record online: 28 JUN 2011 | DOI: 10.1111/j.1742-4658.2011.08207.x

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      We show that there are two different modes of interaction between the heavy and light chain variable domains of immunoglobulins that affect the conformation of the binding site and are related to the type of recognized antigen. This finding is clearly relevant for antibody library design and humanization experiments.

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      Selection of a CXCR4 antagonist from a human heavy chain CDR3-derived phage library (pages 2867–2878)

      Andy Chevigné, Aurélie Fischer, Julie Mathu, Manuel Counson, Nadia Beaupain, Jean-Marc Plesséria, Jean-Claude Schmit and Sabrina Deroo

      Version of Record online: 27 JUN 2011 | DOI: 10.1111/j.1742-4658.2011.08208.x

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      In contrast to negative selection campaigns with random peptide libraries on a peptide corresponding to the second extracellular loop of CXCR4, a single campaign with a human heavy chain CDR3 library resulted in one CXCR4 antagonist (IC50 = 23 μM). Strength and efficacy of the HCDR3 libraries reside in the combination of multiple size peptides and naturally biased sequence variation.

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      Crystal structures of open and closed forms of d-serine deaminase from Salmonella typhimurium – implications on substrate specificity and catalysis (pages 2879–2891)

      Sakshibeedu Rajegowda Bharath, Shveta Bisht, Handanhal Subbarao Savithri and Mattur Ramabhadrashastry Narasimha Murthy

      Version of Record online: 12 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08210.x

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      Salmonella typhimurium D-Serine deaminase (StDSD) is a Foldtype II PLP dependent enzyme that catalyzes degradation of D-Ser to pyruvate and ammonia. In this manuscript, we describe the structures of wild-type and selenomethionine labelled StDSD determined at 2.4Å and 1.9Å resolutions, respectively. These structures provide information on the active site geometry and residues that might be important for catalysis.

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      A short-chain dehydrogenase involved in terpene metabolism from Zingiber zerumbet (pages 2892–2900)

      Sho Okamoto, Fengnian Yu, Hisashi Harada, Toshihide Okajima, Jun-ichiro Hattan, Norihiko Misawa and Ryutaro Utsumi

      Version of Record online: 5 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08211.x

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      The rhizome oil of Zingiber zerumbet Smith contains an exceptionally high content of sesquiterpenoids with zerumbone. In this paper, we isolated a cDNA clone (ZSD1) that encodes an alcohol dehydrogenase responsible for the final step of the zerumbone biosynthesis. Consistent with its proposed flexible substrate-binding pocket, ZSD1 also converts borneol to camphor, displaying its bisubstrate feature.

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      Novel suppression mechanism operating in early phase of adipogenesis by positive feedback loop for enhancement of cyclooxygenase-2 expression through prostaglandin F receptor mediated activation of MEK/ERK-CREB cascade (pages 2901–2912)

      Toshiyuki Ueno and Ko Fujimori

      Version of Record online: 28 JUN 2011 | DOI: 10.1111/j.1742-4658.2011.08213.x

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      PGF2α suppresses the progression of the early phase of adipogenesis by enhancing the binding of CREB to the COX-2 promoter via FP receptor-activated MEK/ERK cascade. Thus, PGF2α forms a positive feedback loop that coordinately suppresses the early phase of adipogenesis through the increased production of anti-adipogenic PGF2α and PGE2.

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      An alternative isomerohydrolase in the retinal Müller cells of a cone-dominant species (pages 2913–2926)

      Yusuke Takahashi, Gennadiy Moiseyev, Ying Chen, Olga Nikolaeva and Jian-Xing Ma

      Version of Record online: 1 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08216.x

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      An RPE-specific 65 kDa protein (RPE65) is an isomerohydrolase, a key enzyme for 11—cis retinal recycling. It was proposed that there is an alternative RPE-independent 11—cis retinal recycling system in the cone-dominant retina. This study identified and characterized a novel isomerohydrolase, RPE65c, in the retinal Müller cells of cone-dominant zebrafish, but not in the RPE.

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      Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau (pages 2927–2937)

      Alessia Usardi, Amy M. Pooler, Anjan Seereeram, C. Hugh Reynolds, Pascal Derkinderen, Brian Anderton, Diane P. Hanger, Wendy Noble and Ritchie Williamson

      Version of Record online: 7 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08218.x

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      Systems biology has received ever increasing interest during the last decade. In this review we focus on applications of systems biology approaches that have been employed to shed light on biochemical functions and mechanisms previously unknown. We point out which computational and experimental methods are employed most frequently and which trends in systems biology research can be observed.

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      Affinity and kinetics of proprotein convertase subtilisin/kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells (pages 2938–2950)

      Seyed A. Mousavi, Knut E. Berge, Trond Berg and Trond P. Leren

      Version of Record online: 18 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08219.x

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      Dissociation of ([125I]-TC-PCSK9) from HepG2 cells was biphasic with two kinetic components. The rate of dissociation of ([125I]-TC-PCSK9) was accelerated by the presence of unlabeled PCSK9. Lowering the pH of dissociation medium from 7.4 to 6.2 resulted in a decrease in the rate of dissociation of ([125I]-TC-PCSK9) and converted the biphasic dissociation curve to a monophasic curve.

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      Multi-targeted activity of maslinic acid as an antimalarial natural compound (pages 2951–2961)

      Carlos Moneriz, Jordi Mestres, José M. Bautista, Amalia Diez and Antonio Puyet

      Version of Record online: 7 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.08220.x

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      We have investigated the molecular targets in Plasmodium falciparum for maslinic acid, a natural triterpene compound showing antimalarial activity. Combined protease inhibition assays and in silico target screening suggested a multi-targeted action this compound, by inhibition of PfSUB1 maturation serine protease as well as other metallo- and cysteine proteases and phospholipase A2.

  3. Author Index

    1. Top of page
    2. Review Articles
    3. Original Articles
    4. Author Index
    1. You have free access to this content
      Author index (page 2962)

      Version of Record online: 28 JUL 2011 | DOI: 10.1111/j.1742-4658.2011.07852.x

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