Dopamine D4 receptor oligomerization – contribution to receptor biogenesis (pages 1333–1344)
Kathleen Van Craenenbroeck, Dasiel O. Borroto-Escuela, Wilber Romero-Fernandez, Kamila Skieterska, Pieter Rondou, Béatrice Lintermans, Peter Vanhoenacker, Kjell Fuxe, Francisco Ciruela and Guy Haegeman
Article first published online: 10 MAR 2011 | DOI: 10.1111/j.1742-4658.2011.08052.x
We investigated the oligomerization properties of three polymorphic repeat variants of the hD4.xR by immunoblot analysis, co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) studies. We demonstrate that hD4.xR oligomerization occurs in the endoplasmic reticulum and plays a role in receptor biogenesis. Furthermore the number of repeats is important for the efficiency and affinity to form oligomers.