Probing the reactivity of different forms of azurin by flavin photoreduction (pages 1506–1521)
Sharmini Alagaratnam, Nico J. Meeuwenoord, José A. Navarro, Manuel Hervás, Miguel A. De la Rosa, Maren Hoffmann, Oliver Einsle, Marcellus Ubbink and Gerard W. Canters
Article first published online: 22 MAR 2011 | DOI: 10.1111/j.1742-4658.2011.08067.x
Replacing Cu-ligand His117 by glycine in azurin leads to increased flexibility of the ligand loop and a gap in the Cu coordination shell that is partly filled with water molecules. Flash photolysis experiments show that His117 acts as an electronic conduit for electron transfer; the water molecules in the H117G variant are less efficient in this respect.