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The FEBS Journal

Cover image for Vol. 279 Issue 1

January 2012

Volume 279, Issue 1

Pages 1–192

  1. Minireview Series

    1. Top of page
    2. Minireview Series
    3. Minireview
    4. Original Articles
    5. Author index
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      Src family kinases in the nervous system (page 1)

      Xian-Min Yu and Bradley R. Groveman

      Version of Record online: 5 DEC 2011 | DOI: 10.1111/j.1742-4658.2011.08423.x

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      Src family kinases (SFKs) are key factors in the process of coupling signals from the cell surface to intracellular machinery and critically involved in the regulation of many neural functions mediated through growth factors, G-protein-coupled receptors or ligand-gated ion channels. The three minireviews here focus on recent findings dealing with the regulation of N-methyl-d-aspartate (NMDA) receptors by SFKs.

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  2. Minireview

    1. Top of page
    2. Minireview Series
    3. Minireview
    4. Original Articles
    5. Author index
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      Dysregulated Src upregulation of NMDA receptor activity: a common link in chronic pain and schizophrenia (pages 2–11)

      Michael W. Salter and Graham M. Pitcher

      Version of Record online: 5 DEC 2011 | DOI: 10.1111/j.1742-4658.2011.08390.x

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      Upregulation of N-methyl d-aspartate (NMDA) receptor function by the nonreceptor protein tyrosine kinase Src has been implicated in physiological plasticity at glutamatergic synapses. Here, we highlight recent findings which suggest that aberrant Src regulation of NMDA receptors may also be implicated in pathophysiological conditions including chronic pain and positive symptoms of schizophrenia.

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      Regulation of NMDA receptors by the tyrosine kinase Fyn (pages 12–19)

      Catherine H. Trepanier, Michael F. Jackson and John F. MacDonald

      Version of Record online: 5 DEC 2011 | DOI: 10.1111/j.1742-4658.2011.08391.x

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      In the second minireview of this series, we outline the mechanisms by which Fyn regulates the phosphorylation and trafficking of N-methyl d-aspartate (NMDA) receptor subunits, and describe how this regulation mediates signaling from ethanol and G-protein-coupled receptors. In conclusion, we highlight studies where Fyn modulates the threshold for synaptic plasticity and different types of learning.

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      The regulation of N-methyl-d-aspartate receptors by Src kinase (pages 20–28)

      Bradley R. Groveman, Shuang Feng, Xiao-Qian Fang, Melissa Pflueger, Shuang-Xiu Lin, Ewa A. Bienkiewicz and XianMin Yu

      Version of Record online: 5 DEC 2011 | DOI: 10.1111/j.1742-4658.2011.08413.x

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      The N-methyl d-aspartate (NMDA) receptor is complexed with an Src family kinase (SFK) signaling complex consisting of SFKs, the SFK-activating phosphatase PTPalpha, and the SFK-inactivating kinase Csk. The activity of Src may be regulated by its stability. The SH2 and SH3 domains may act not only to constrain the activation of Src, but also to promote the activity of Src.

  3. Original Articles

    1. Top of page
    2. Minireview Series
    3. Minireview
    4. Original Articles
    5. Author index
    1. You have free access to this content
      8-Oxoguanine in a quadruplex of the human telomere DNA sequence (pages 29–39)

      Michaela Vorlícková, Martin Tomasko, Andras J. Sagi, Klara Bednarova and Janos Sagi

      Version of Record online: 11 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08396.x

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      8-Oxoguanine, a major in vivo oxidation product in nucleic acids, if formed in the G-quadruplex structures capping the human chromosomes causes significant enthalpic destabilization in any G-position of the quadruplex, as studied here with dG3(TTAG3)3. The conformational rearrangement observed with the unmodified quadruplex in K+ was inhibited with most 8-oxoguanine-containing structures implicating biological relevancies of the results.

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      Photophysical diversity of two novel cyanobacteriochromes with phycocyanobilin chromophores: photochemistry and dark reversion kinetics (pages 40–54)

      Yu Chen, Juan Zhang, Juan Luo, Jun-Ming Tu, Xiao-Li Zeng, Jie Xie, Ming Zhou, Jing-Quan Zhao, Hugo Scheer and Kai-Hong Zhao

      Version of Record online: 11 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08397.x

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      GAF domains from cyanobacteriochromes could be attached covalently to phycobilins. After GAF1 of RGS from Synechocystis, GAF1 and GAF3 of AphC from Nostoc binds to phycocyanobilin, the resulting chromoproteins are photochromic which regulates the autophosphorylation activity of cyanobacteriochromes. Chromophorylated GAF3 of AphC shows a novel red-orange photochromism; the unstable state reverts very rapidly (τ∼20 s) back to the thermostable Z-state.

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      Inhibition of type IA topoisomerase by a monoclonal antibody through perturbation of DNA cleavage–religation equilibrium (pages 55–65)

      Majety Naga Leelaram, Anuradha Gopal Bhat, Shivanand Manjunath Hegde, Ramanathapuram Manjunath and Valakunja Nagaraja

      Version of Record online: 11 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08398.x

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      Type IA DNA topoisomerases from bacteria are potential drug targets for which inhibitors are being discovered now. We identified a monoclonal antibody, 2F3G4, which specifically inhibited mycobacterial topoisomerase I in nanomolar range. The antibody stimulated enzyme-mediated DNA cleavage and elevated protein-DNA covalent adducts. The mode of inhibition opens up avenues to develop new, small molecule inhibitors for bacterial topoisomerase I.

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      Identification of Dp71e, a new dystrophin with a novel carboxy-terminal end (pages 66–77)

      Abril Saint Martín, Jorge Aragón, Francisco Depardon-Benítez, Alejandra Sánchez-Trujillo, Guillermo Mendoza-Hernández, Victor Ceja and Cecilia Montañez

      Version of Record online: 17 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08399.x

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      Sodium 2-propenyl-thiosulfate (2-PTS) is a garlic water-soluble organo-sulfane sulfur compound which spontaneously reacts with reduced glutathione (GSH) at physiological pH, leading to the formation of glutathionyl/derivative S-allyl-mercapto-glutathione (GSSA), radicals and peroxyl species, which are able to induce inhibition of enzymes with sulphydril active centres. The effects of GSSA on the cell growth of HuT 78 cells were also investigated observing an antiproliferative effect with a blockage in G2/M phase.

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      Role of charged residues in stabilization of Pyrococcus horikoshii CutA1, which has a denaturation temperature of nearly 150 °C (pages 78–90)

      Yoshinori Matsuura, Michiyo Takehira, Masahide Sawano, Kyoko Ogasahara, Tomoyuki Tanaka, Hitoshi Yamamoto, Naoki Kunishima, Etsuko Katoh and Katsuhide Yutani

      Version of Record online: 14 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08400.x

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      The CutA1 protein from Pyrococcus horikoshii (PhCutA1), a hyperthermophile, has an unusually high content of charged residues and an unusually high denaturation temperature of 150 °C. To elucidate the role of ion-ion interactions in protein stability, the mutant proteins of PhCutA1 in which charged residues were substituted by non-charged residues were comprehensively examined.

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      Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII (pages 91–99)

      Jingyuan Wei, Hongsheng Ouyang, Yuhui Wang, Daxin Pang, Nathan X. Cong, Tiedong Wang, Bingfeng Leng, Dong Li, Xiaoping Li, Rong Wu, Yu Ding, Fei Gao, Yanhong Deng, Bo Liu, Ziyi Li, Liangxue Lai, Haihua Feng, George Liu and Xuming Deng

      Version of Record online: 23 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08401.x

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      We generated transgenic miniature swine expressing human ApoCIII through the transfection of somatic cells combined with nuclear transfer. Human ApoCIII expression was detected in the liver and intestine in transgenic pigs. These animals also exhibited increased plasma triglyceride and delayed clearance. This model could be of great value for studies on hypertriglyceridemia in relation with atherosclerosis.

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      Biosynthesis of UDP-glucuronic acid and UDP-galacturonic acid in Bacillus cereus subsp. cytotoxis NVH 391-98 (pages 100–112)

      Bryan Broach, Xiaogang Gu and Maor Bar-Peled

      Version of Record online: 11 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08402.x

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      We report the functional identification of a UDP-glucose 6-dehydrogenase (UGlcDH) and UDP-glucuronic acid 4-epimerase (UGlcAE) in Bacillus cereus. The enzymes can also utilize TDP-glucose and TDP-glucuronic acid, but the epimerase demonstrated specificity not seen in similar bacterial epimerases. The operon is possibly involved in pathways for cytosol acidification and formation of secreted acidic-glycans aimed at protecting the pathogen from alkaline conditions.

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      Substrate specificity of the deazaflavin-dependent nitroreductase from Mycobacterium tuberculosis responsible for the bioreductive activation of bicyclic nitroimidazoles (pages 113–125)

      Meera Gurumurthy, Tathagata Mukherjee, Cynthia S. Dowd, Ramandeep Singh, Pornwaratt Niyomrattanakit, Jo Ann Tay, Amit Nayyar, Yong Sok Lee, Joseph Cherian, Helena I. Boshoff, Thomas Dick, Clifton E. Barry III and Ujjini H. Manjunatha

      Version of Record online: 14 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08404.x

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      PA-824, a bicyclic-nitroimidazole in clinical development against tuberculosis, is bioreductively activated by a deazaflavin (F420) dependent nitroreductase (Ddn). Substrate and stereospecificity of Ddn is directed by the presence of a long hydrophobic tail. (R)-PA-824 binds Ddn with the same affinity as the active (S)-PA-824, despite not being turned over. Docking studies suggest that (R)-PA-824 likely binds non-productively to the enzyme.

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      pH dependence of listeriolysin O aggregation and pore-forming ability (pages 126–141)

      Andrej Bavdek, Rok Kostanjšek, Valeria Antonini, Jeremy H. Lakey, Mauro Dalla Serra, Robert J. C. Gilbert and Gregor Anderluh

      Version of Record online: 25 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08405.x

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      The effect of pH on Listeriolysin O aggregation and pore forming ability was studied. We show that its structure is not detectably affected by pH at room temperature. We observed slightly altered hemolytic and permeabilising activities at different pH values. However, alkaline pH and elevated temperatures caused rapid aggregation and loss of function. The aggregates have the biophysical properties of amyloid.

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      Template-independent DNA synthesis activity associated with the reverse transcriptase of the long terminal repeat retrotransposon Tf1 (pages 142–153)

      Iris Oz-Gleenberg, Eytan Herzig and Amnon Hizi

      Version of Record online: 25 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08406.x

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      Reverse transcriptases (RTs) possess a non-templated addition (NTA) activity while synthesizing DNA with blunt-ended DNA primer-templates. The RT of the retrotransposon Tf1 has an NTA activity that is substantially higher than that of human immunodeficiency virus type-1 or murine leukemia virus RTs. This feature can be explained by the substantially lower KM value for the incoming dNTP of Tf1 RT.

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      Oxidative species and S-glutathionyl conjugates in the apoptosis induction by allyl thiosulfate (pages 154–167)

      Ridvan Nepravishta, Renato Sabelli, Egidio Iorio, Laura Micheli, Maurizio Paci and Sonia Melino

      Version of Record online: 17 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08407.x

      Thumbnail image of graphical abstract

      Sodium 2-propenyl-thiosulfate (2-PTS) is a garlic water-soluble organo-sulfane sulfur compound which spontaneously reacts with reduced glutathione (GSH) at physiological pH, leading to the formation of glutathionyl/derivative S-allyl-mercapto-glutathione (GSSA), radicals and peroxyl species, which are able to induce inhibition of enzymes with sulphydril active centres. The effects of GSSA on the cell growth of HuT 78 cells were also investigated observing an antiproliferative effect with a blockage in G2/M phase.

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      A hemolymph major anionic peptide, HemaP, motivates feeding behavior in the sweetpotato hornworm, Agrius convolvuli (pages 168–179)

      Nobukatsu Morooka, Shinji Nagata, Koji Shirai, Kenji Kiguchi and Hiromichi Nagasawa

      Version of Record online: 23 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08408.x

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      An insect feeding-modulating hemolymph peptide hemolymph major anionic peptide (HemaP) has been purified and identified from the sweetpotato hornworm, Agrius convolvuli. Although structural characteristics of A. convolvuli HemaP was similar to those of HemaPs from other lepidopteran species including the silkworm Bombyx mori, recombinant A. convolvuli and B. mori HemaPs exhibited no cross-reactivity.

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      Identification and analysis of functionally important amino acids in human purinergic 12 receptor using a Saccharomyces cerevisiae expression system (pages 180–191)

      Vita Ignatovica, Kaspars Megnis, Maris Lapins, Helgi B. Schiöth and Janis Klovins

      Version of Record online: 23 NOV 2011 | DOI: 10.1111/j.1742-4658.2011.08410.x

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      To explore the role of positions 181, 256, 265, and 280 in functional activity of the purinergic 12 receptor, we generated four randomised libraries of the receptor and demonstrated alterations in signal transduction caused by amino acid substitutions. Results of our findings were analysed by QSAR and by modelling the 3D structure of the receptor.

  4. Author index

    1. Top of page
    2. Minireview Series
    3. Minireview
    4. Original Articles
    5. Author index
    1. You have free access to this content
      Author index (page 192)

      Version of Record online: 13 DEC 2011 | DOI: 10.1111/j.1742-4658.2011.08321.x

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