Hemodynamic responses in amygdala and hippocampus distinguish between aversive and neutral cues during Pavlovian fear conditioning in behaving rats
Article first published online: 22 NOV 2012
© 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 37, Issue 3, pages 498–507, February 2013
How to Cite
McHugh, S. B., Marques-Smith, A., Li, J., Rawlins, J. N. P., Lowry, J., Conway, M., Gilmour, G., Tricklebank, M. and Bannerman, D. M. (2013), Hemodynamic responses in amygdala and hippocampus distinguish between aversive and neutral cues during Pavlovian fear conditioning in behaving rats. European Journal of Neuroscience, 37: 498–507. doi: 10.1111/ejn.12057
- Issue published online: 3 FEB 2013
- Article first published online: 22 NOV 2012
- Manuscript Accepted: 13 OCT 2012
- Manuscript Revised: 12 OCT 2012
- Manuscript Received: 27 JUL 2012
- Wellcome Trust. Grant Numbers: 074385, 087736
- Biotechnology and Biological Sciences Research Council (BBSRC)
Fig. S1. Behavioral (A) and tissue oxygen (TO2) responses in the basolateral amygdala (BLA; B), dorsal hippocampus (dHPC; C) and ventral hippocampus (vHPC; D) during pre-exposure, training and extinction of Pavlovian fear conditioning. Columns 1–4 show mean responses to the five CS− and five CS+ presentations during each day of phase I (pre-exposure, training I, training II and extinction, respectively). Columns 5 and 6 show responses for the first and last CS+ and CS− trials of extinction (i.e. column 5: CS−1 vs. CS+1; column 6: CS−5 vs. CS+5). (A) Mean (±SEM) percentage freezing during the 30 s of the CS− (blue bars) or CS+ (red bars). (B–D) Mean (±SEM) TO2 change from baseline (ΔTO2) during the 30 s of CS− (blue traces) and CS+ (red traces) in 2-s timebins. n.s., no significant difference in paired t-test, or no main effect of CS or CS × timebin interaction; *significant difference in paired t-test (P < 0.05) or main effect of CS (P < 0.05); #CS × timebin interaction (P < 0.05).
Fig. S2. Freezing responses to the CS− (blue) and CS+ (red) during the last five trials of training. There was no evidence of discriminative freezing (i.e. higher freezing during CS+ trials) during any of the training trials.
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