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Fig. S1. C57BL/6 mice avoid an environment paired with ethanol withdrawal. After 4 days of exposure to Lieber DeCarli's Ethanol Liquid Diet (replacing all rodent chow and water), the diet was removed and replaced with a control liquid diet at 8 h prior to a 5-min conditioning session for C57BL/6 mice (no drug injections) (n = 8). At 1 h after conditioning, the ethanol diet was reintroduced and the control diet removed. This cycle continued for 4 days before the mice were given 1 week to recover with regular rodent chow and water. A 10 min conditioning test revealed a significant conditioned place aversion to the ethanol withdrawal-paired environment vs. the neutral environment (t1,7 = 2.58, = 0.036). Data represent mean + SEM of time spent in the environments.

Fig. S2. C57BL/6 mice show no baseline preferences in our place conditioning paradigm. Mice received a saline injection prior to a 5 min conditioning session in one of two distinct conditioning environments (see Materials and methods) (n = 15). This continued for 8 days (with exposures to alternating conditioning environments) after which the mice were given 1 week prior to testing. A 10 min conditioning test revealed no significant preference or aversion to either the black or white conditioning environments (t1,14 = 0.532, = 0.603). Data represent mean + SEM of time spent in the environments.

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