Infusion of brain-derived neurotrophic factor into the ventral tegmental area switches the substrates mediating ethanol motivation
Article first published online: 21 DEC 2012
© 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 37, Issue 6, pages 996–1003, March 2013
How to Cite
Ting-A-Kee, R., Vargas-Perez, H., Bufalino, M.-R., Bahi, A., Dreyer, J.-L., Tyndale, R. F. and van der Kooy, D. (2013), Infusion of brain-derived neurotrophic factor into the ventral tegmental area switches the substrates mediating ethanol motivation. European Journal of Neuroscience, 37: 996–1003. doi: 10.1111/ejn.12105
- Issue published online: 18 MAR 2013
- Article first published online: 21 DEC 2012
- Manuscript Accepted: 25 NOV 2012
- Manuscript Revised: 26 OCT 2012
- Manuscript Received: 17 APR 2012
Fig. S1. C57BL/6 mice avoid an environment paired with ethanol withdrawal. After 4 days of exposure to Lieber DeCarli's Ethanol Liquid Diet (replacing all rodent chow and water), the diet was removed and replaced with a control liquid diet at 8 h prior to a 5-min conditioning session for C57BL/6 mice (no drug injections) (n = 8). At 1 h after conditioning, the ethanol diet was reintroduced and the control diet removed. This cycle continued for 4 days before the mice were given 1 week to recover with regular rodent chow and water. A 10 min conditioning test revealed a significant conditioned place aversion to the ethanol withdrawal-paired environment vs. the neutral environment (t1,7 = 2.58, P = 0.036). Data represent mean + SEM of time spent in the environments.
Fig. S2. C57BL/6 mice show no baseline preferences in our place conditioning paradigm. Mice received a saline injection prior to a 5 min conditioning session in one of two distinct conditioning environments (see Materials and methods) (n = 15). This continued for 8 days (with exposures to alternating conditioning environments) after which the mice were given 1 week prior to testing. A 10 min conditioning test revealed no significant preference or aversion to either the black or white conditioning environments (t1,14 = 0.532, P = 0.603). Data represent mean + SEM of time spent in the environments.
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