Fig. S1. C57BL/6 mice avoid an environment paired with ethanol withdrawal. After 4 days of exposure to Lieber DeCarli's Ethanol Liquid Diet (replacing all rodent chow and water), the diet was removed and replaced with a control liquid diet at 8 h prior to a 5-min conditioning session for C57BL/6 mice (no drug injections) (n = 8). At 1 h after conditioning, the ethanol diet was reintroduced and the control diet removed. This cycle continued for 4 days before the mice were given 1 week to recover with regular rodent chow and water. A 10 min conditioning test revealed a significant conditioned place aversion to the ethanol withdrawal-paired environment vs. the neutral environment (t1,7 = 2.58, = 0.036). Data represent mean + SEM of time spent in the environments.

Fig. S2. C57BL/6 mice show no baseline preferences in our place conditioning paradigm. Mice received a saline injection prior to a 5 min conditioning session in one of two distinct conditioning environments (see Materials and methods) (n = 15). This continued for 8 days (with exposures to alternating conditioning environments) after which the mice were given 1 week prior to testing. A 10 min conditioning test revealed no significant preference or aversion to either the black or white conditioning environments (t1,14 = 0.532, = 0.603). Data represent mean + SEM of time spent in the environments.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.