Fig. S1. Synaptosomes isolated from the forebrain of wild-type animals are functionally active. A – The association of [3H]-glutamate over time with isolated synaptosomes in the presence of sodium (closed squares) or choline (open triangles). B – The association of [3H]-glutamate with isolated synaptosomes in the presence (open triangles) and absence (closed squares) of 300 μm TBOA. C – Glutamate uptake over time in the absence (closed squares) and presence (open triangles) of 250 μg/ml of saponin. D – Histograms showing the average glutamate uptake into synaptosomes over 30 secs at 0 ºC, 22 ºC and 37ºC. ±SEM; n = 3.

Fig. S2. Saturation analysis of glutamate uptake into the synaptosomal preparations. A – Nonlinear regression analysis demonstrating the association of [3H]-glutamate with synaptosomes isolated from wild-type forebrain in the presence of increasing concentrations of glutamate (closed squares). ±SEM; n = 3 B – Double-reciprocal Lineweaver-Burk plot linearizing rate of glutamate uptake with increasing concentration of glutamate.

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