Rare contacts between synapses and microglial processes containing high levels of Iba1 and actin – a postembedding immunogold study in the healthy rat brain
Article first published online: 17 APR 2013
© 2013 Federation of European Neuroscience Societies and John Wiley & Sons Ltd
European Journal of Neuroscience
Volume 38, Issue 1, pages 2030–2040, July 2013
How to Cite
Sogn, C. J. L., Puchades, M. and Gundersen, V. (2013), Rare contacts between synapses and microglial processes containing high levels of Iba1 and actin – a postembedding immunogold study in the healthy rat brain. European Journal of Neuroscience, 38: 2030–2040. doi: 10.1111/ejn.12213
- Issue published online: 1 JUL 2013
- Article first published online: 17 APR 2013
- Manuscript Accepted: 7 MAR 2013
- Manuscript Revised: 6 MAR 2013
- Manuscript Received: 14 DEC 2012
- Research Council of Norway
- Letten Saugstad Foundation
Data S1. Lowicryl postembedding immunogold microscopy.
Fig. S1. Example of a microglial process outlined (blue dotted line) by use of ImageJ (http://rsb.info.nih.gov/ij/).
Fig. S2. The Iba1 antibodies produced selective labelling of microglia.
Fig. S3. Postembedding Iba1 immunogold labelling of microglia in the frontal cortex from Lowicryl embedded tissue.
Fig. S4. Low power electron micrograph showing an overview of the frontal cortex neuropil.
Table S1. Frequency of microglial contacts with synapses as observed with 10-nm gold particles.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.