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Data S1. Lowicryl postembedding immunogold microscopy.

Fig. S1. Example of a microglial process outlined (blue dotted line) by use of ImageJ (http://rsb.info.nih.gov/ij/).

Fig. S2. The Iba1 antibodies produced selective labelling of microglia.

Fig. S3. Postembedding Iba1 immunogold labelling of microglia in the frontal cortex from Lowicryl embedded tissue.

Fig. S4. Low power electron micrograph showing an overview of the frontal cortex neuropil.

Table S1. Frequency of microglial contacts with synapses as observed with 10-nm gold particles.

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