SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
ejn12524-sup-0001-FigS1-S2.pdfapplication/PDF2592K

Fig. S1. Microphotographs showing location of all VPM (A) and Po (B) BDA injection sites. The number of labeled axons counted as they cross the striato-pallial border is indicated for each experiment. Scale bars: 100 μm. (C, D) Quantification of VPM and Po total axonal length and boutons following ION section. Values: mean ± SEM. (E, F) Total individual axons effectively labeled by the BDA microinjections was estimated by counting the axonal trunks (arrowheads) at their point of crossing of the striato-pallial border. Cytochrome oxidase counterstain. Scale bars E and F: 500 and 100 μm respectively. (G) High-magnification of labeled terminal cortical branches of VPM axons containing bouton-like varicosities. Scale bar: 5 μm.

Fig. S2. C-Fos expression reflects genuine activation of Po and VPM neurons. (A) Cytochrome oxidase staining showing the distribution of the distinct cortical layers across bins: bin 1 corresponds to L1, bin 2 and 3 to L2/3, bin 4 and 5 to L4, bin 6 to L5A, bin 7 to L5B and bin 8, 9 and 10 to L6. Scale bar: 100 μm. (B) Disinhibition of corticothalamic neurons by bicuculline injection in deep layers of S1BF induces ipsilateral expression of c-Fos in Po neurons. Scale bar: 100 μm. (C) L4 and VPM neurons are strongly activated by exploration of the environment in unclipped mice. Blue arrowheads delineate a barrel. Quantification of bin- (empty circles) and layer-specific (bars) fold-changes in c-Fos+ neuron numbers in response to EE with all whiskers (unclipped mouse). Graph bars are color-coded to indicate the percentage of c-Fos+ neurons in each corresponding layer for active whiskers. Scale bars: 100 μm. (D) Dual whisker environmental exploration and noxious stimulation induces c-Fos expression in SpVc neurons. Scale bars: 100 μm.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.