GABAA receptors can initiate the formation of functional inhibitory GABAergic synapses (pages 3146–3158)
Celine Fuchs, Karine Abitbol, Jemima J. Burden, Audrey Mercer, Laura Brown, Jonathan Iball, F. Anne Stephenson, Alex M. Thomson and Jasmina N. Jovanovic
Article first published online: 5 AUG 2013 | DOI: 10.1111/ejn.12331
GABAA receptors initiate rapid formation of synaptic contacts in heterologous co-culture systems. These contacts are stable, as assessed by live cell imaging; they are active, as determined by uptake of a fluorescently-labelled synaptotagmin vesicle-luminal domain-specific antibody, and they support spontaneous and action-potential-driven postsynaptic GABAergic currents. Ultrastructural analysis confirmed characteristics typical of active synapses.