Urinary profile of catecholamines and metabolites in Parkinson patients with deep brain stimulation

Authors

  • J. Guimarães,

    Corresponding author
    1. Neurology Department, Hospital de São João, Porto, Portugal
    2. Neurology and Neurosurgery Unit of Clinical Neurosciences and Mental Health Department, Faculty of Medicine, University of Porto, Porto, Portugal
    3. Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
    • Correspondence: Joana Guimarães, Neurology Department, Faculty of Medicine, University of Porto, Hospital de São João, Alameda Professor Hernâni Monteiro, 4200 Porto, Portugal (tel./fax: 225511200; e-mail: jguimraes9@hotmail.com).

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  • M. A. Vieira-Coelho,

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
    2. Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
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  • E. Moura,

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
    2. Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
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  • J. Afonso,

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
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  • M. J. Rosas,

    1. Neurology Department, Hospital de São João, Porto, Portugal
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  • R. Vaz,

    1. Neurology and Neurosurgery Unit of Clinical Neurosciences and Mental Health Department, Faculty of Medicine, University of Porto, Porto, Portugal
    2. Department of Neurosurgery, Hospital de São João, Porto, Portugal
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  • C. Garrett

    1. Neurology Department, Hospital de São João, Porto, Portugal
    2. Neurology and Neurosurgery Unit of Clinical Neurosciences and Mental Health Department, Faculty of Medicine, University of Porto, Porto, Portugal
    3. Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
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Abstract

Background and purpose

Deep brain stimulation of the subthalamic nucleus (DBS-STN) is thought to continuously alter the activity of STN neurons in Parkinson's disease (PD). A chronic decrease in the levodopa dose with continuous STN stimulation may induce plastic neuronal changes.

Objective

The objective of this work was to study urinary excretion of catecholamines in patients with PD before and after DBS-STN.

Methods

Twenty-three patients were submitted to DBS-STN, and evaluated before and after surgery with respect to catecholamines and metabolites in 24-h urine measured by high-performance liquid chromatography with electrochemical detection.

Results

Of the 23 patients evaluated, a significant decrease of about 60% in the urinary excretion of l-3,4-dihydroxyphenylalanine (l-DOPA; in nmol/mg creatinine/24 h) was observed 1 week after DBS-STN. Moreover, in 17 patients with a follow-up of 8 weeks after surgery, there was a further 50% decrease in urinary l-DOPA levels, dropping to about 75% of the values before surgery. There was also a significant decrease in dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) levels 1 week after DBS-STN that was no longer present 8 weeks after. A significant increase in the DA/l-DOPA ratio was observed 1 week after surgery, with a further increase 8 weeks after surgery.

Conclusion

After DBS-STN, the DA/l-DOPA ratio, an indirect measure of DA synthesis, increased. These results show that DBS-STN may improve the efficacy of oral levodopa.

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