Influence of pH on inhibition of Streptococcus mutans by Streptococcus oligofermentans
Version of Record online: 25 NOV 2013
© 2013 Eur J Oral Sci
European Journal of Oral Sciences
Volume 122, Issue 1, pages 57–61, February 2014
How to Cite
Influence of pH on inhibition of Streptococcus mutans by Streptococcus oligofermentans. Eur J Oral Sci 2014; 122: 57–61. © 2013 Eur J Oral Sci, , , , , , .
- Issue online: 9 JAN 2014
- Version of Record online: 25 NOV 2013
- Manuscript Accepted: 10 OCT 2013
- hydrogen peroxide;
- pH ;
- Streptococcus mutans ;
- Streptococcus oligofermentans
Streptococcus oligofermentans is a novel strain of oral streptococcus that can specifically inhibit the growth of Streptococcus mutans. The aims of this study were to assess the growth of S. oligofermentans and the ability of S. oligofermentans to inhibit growth of Streptococcus mutans at different pH values. Growth inhibition was investigated in vitro using an interspecies competition assay. The 4-aminoantipyine method was used to measure the initial production rate and the total yield of hydrogen peroxide in S. oligofermentans. S. oligofermentans grew best at pH 7.0 and showed the most pronounced inhibitory effect when it was inoculated earlier than S. mutans. In terms of the total yield and the initial production rate of hydrogen peroxide by S. oligofermentans, the effects of the different culture pH values were as follows: pH 7.0 > 6.5 > 6.0 > 7.5 > 5.5 = 8.0 (i.e. there was no significant difference between pH 5.5 and pH 8.0). Environmental pH and the sequence of inoculation significantly affected the ability of S. oligofermentans to inhibit the growth of S. mutans. The degree of inhibition may be attributed to the amount of hydrogen peroxide produced.