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Appendix S1. Results from the analyses of variance

Table S1. Background information on each of the four populations.

Table S2. Tadpole tolerance to carbaryl (time to death) from populations close and far from agriculture in the absence of a previous sublethal exposure to carbaryl.

Table S3. Survival of tadpoles exposed to 0 ppm of carbaryl in the TTD assay for the Embryo-exposure and hatchling-exposure experiments.

Table S4. Test results from anovas on survival of tadpoles exposed to the lethal concentration during the TTD assay for A) the embryo-exposure experiment, B) the hatchling exposure experiment.

Figure S1. Survival of tadpoles exposed to four sublethal concentrations of carbaryl as embryos (0, 0.07, 0.25, and 0.62 ppm) followed by an exposure to a lethal concentration as tadpoles during the TTD assay.

Figure S2. Survival of tadpoles exposed to four sublethal concentrations of carbaryl as hatchlings (0, 0.07, 0.25, and 0.62 ppm) followed by an exposure to a lethal concentration as tadpoles during the TTD assay.

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