Antagonistic relationships between intron content and codon usage bias of genes in three mosquito species: functional and evolutionary implications
Version of Record online: 24 JUL 2013
© 2013 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 6, Issue 7, pages 1079–1089, November 2013
How to Cite
Behura, S. K., Singh, B. K. and Severson, D. W. (2013), Antagonistic relationships between intron content and codon usage bias of genes in three mosquito species: functional and evolutionary implications. Evolutionary Applications, 6: 1079–1089. doi: 10.1111/eva.12088
- Issue online: 9 OCT 2013
- Version of Record online: 24 JUL 2013
- Manuscript Accepted: 14 JUN 2013
- Manuscript Received: 8 APR 2013
- National Institute of Allergy and Infectious Diseases (NIAD)
- National Institutes of Health
- codon bias;
Genome biology of mosquitoes holds potential in developing knowledge-based control strategies against vectorborne diseases such as malaria, dengue, West Nile, and others. Although the genomes of three major vector mosquitoes have been sequenced, attempts to elucidate the relationship between intron and codon usage bias across species in phylogenetic contexts are limited. In this study, we investigated the relationship between intron content and codon bias of orthologous genes among three vector mosquito species. We found an antagonistic relationship between codon usage bias and the intron number of genes in each mosquito species. The pattern is further evident among the intronless and the intron-containing orthologous genes associated with either low or high codon bias among the three species. Furthermore, the covariance between codon bias and intron number has a directional component associated with the species phylogeny when compared with other nonmosquito insects. By applying a maximum likelihood–based continuous regression method, we show that codon bias and intron content of genes vary among the insects in a phylogeny-dependent manner, but with no evidence of adaptive radiation or species-specific adaptation. We discuss the functional and evolutionary significance of antagonistic relationships between intron content and codon bias.