ANALYTICAL CLINICAL STUDIES
Plasma concentrations of inflammatory markers in previously laminitic ponies
Version of Record online: 19 FEB 2013
© 2012 EVJ Ltd
Equine Veterinary Journal
Volume 45, Issue 5, pages 546–551, September 2013
How to Cite
Wray, H., Elliott, J., Bailey, S. R., Harris, P. A. and Menzies-Gow, N. J. (2013), Plasma concentrations of inflammatory markers in previously laminitic ponies. Equine Veterinary Journal, 45: 546–551. doi: 10.1111/evj.12031
- Issue online: 2 AUG 2013
- Version of Record online: 19 FEB 2013
- Accepted manuscript online: 19 DEC 2012 09:50PM EST
- Manuscript Accepted: 5 DEC 2012
- Manuscript Received: 12 SEP 2012
- tumour necrosis factor-α;
Reasons for performing study
The mechanisms underlying individual animal predisposition to pasture-associated laminitis remain unclear; however, chronic inflammation is implicated.
To identify differences in the inflammatory profile of a group of previously laminitic ponies compared with control animals at pasture in late spring and winter.
Previously laminitic (PL; n = 38 and 42) and nonlaminitic control ponies (NL; n = 41 and 39) were sampled in late spring and winter. Body condition score, height, weight and crest height and thickness were measured. Plasma concentrations of tumour necrosis factor-α, serum amyloid A, haptoglobin, insulin, adiponectin, triglyceride, fibrinogen, interleukin-17, interleukin-4 and interferon-γ were assayed by validated/standard methods. Factors independently associated with each cytokine were determined by multivariate analysis.
Plasma [adiponectin] was significantly influenced by laminitis status, being lower in PL (median [interquartile range] 2.1[1.4–3.2] μg/l) than in NL ponies (3.4 [2.6–4.1] μg/l; P<0.0001). No other cytokines or inflammatory markers were associated with laminitis status. Plasma fibrinogen and serum amyloid A concentrations were significantly (P = 0.04 and P = 0.01) higher in geldings (3.5 [3.0–4.0] g/l; 2.2 [0.5–3.6] mg/l) than in mares (3.0 [3.0–4.0] g/l; 1.5 [0.4–2.1] mg/l) and significantly (P = 0.04 and P<0.001) higher in winter (3.5 [3.0–4.0] g/l; 2.5 [0.9–3.6] mg/l) than in late spring (3.0 [3.0–3.5] g/l; 1.1 [0.3–1.9] mg/l). Serum haptoglobin concentration showed the same significant (P<0.001) seasonal difference (winter 2.1 [1.6–2.6 g/l; late spring 1.8 1.4–2.4 g/l) and was significantly (P = 0.01) inversely associated with weight. Serum interleukin-4 concentration was significantly (P<0.0001) higher in winter (2.0 [1.2–3.0] ng/l) than in late spring (0.0 [0.0–0.0] ng/l). Serum insulin concentration was significantly (P = 0.02) influenced by season (winter 31.7 [9.6–43.5] miu/l; late spring 84.0 [7.0–131.0] miu/l). Plasma triglyceride concentration was significantly (P = 0.02) higher in PL (0.5 [0.3–0.7] mmol/l) than in NL ponies (0.4 [0.2–0.5] mmol/l).
There were significant effects of season, gender and bodyweight on a number of proinflammatory mediators or markers of inflammation. The only marker influenced by laminitis status was adiponectin, and concentrations of this anti-inflammatory marker were lower in previously laminitic animals.
Recurrent laminitis may be associated with reduced anti-inflammatory capacity rather than a proinflammatory state.