EXPERIMENTAL AND BASIC RESEARCH STUDIES
Distribution and persistence of technetium-99 hexamethyl propylene amine oxime-labelled bone marrow-derived mesenchymal stem cells in experimentally induced tendon lesions after intratendinous injection and regional perfusion of the equine distal limb
Version of Record online: 9 APR 2013
© 2013 EVJ Ltd
Equine Veterinary Journal
Volume 45, Issue 6, pages 726–731, November 2013
How to Cite
Sole, A., Spriet, M., Padgett, K. A., Vaughan, B., Galuppo, L. D., Borjesson, D. L., Wisner, E. R. and Vidal, M. A. (2013), Distribution and persistence of technetium-99 hexamethyl propylene amine oxime-labelled bone marrow-derived mesenchymal stem cells in experimentally induced tendon lesions after intratendinous injection and regional perfusion of the equine distal limb. Equine Veterinary Journal, 45: 726–731. doi: 10.1111/evj.12063
- Issue online: 14 OCT 2013
- Version of Record online: 9 APR 2013
- Accepted manuscript online: 18 FEB 2013 04:01AM EST
- Manuscript Accepted: 27 JAN 2013
- Manuscript Received: 2 SEP 2012
- mesenchymal stem cell;
- regional limb perfusion;
- superficial digital flexor tendon;
- experimental injury;
Reasons for performing study
Intralesional (i.l.) injection is currently the most commonly used technique for stem cell therapy in equine tendon injury. A comparison of different techniques of injection of mesenchymal stem cells for the treatment of tendon lesions is required.
We hypothesised that vascular perfusion of the equine distal limb with mesenchymal stem cells (MSCs) would result in preferential distribution of MSCs to acute tendon injuries.
In vivo experimental study.
Lesions were surgically induced in forelimb superficial digital flexor tendons of 8 horses. Three or 10 days after lesion induction, technetium-99 hexamethyl propylene amine oxime-labelled MSCs were injected via i.v. or intra-arterial (i.a.) regional limb perfusion (RLP) at the level of the distal antebrachium and compared to i.l. injection. Mesenchymal stem cell persistence and distribution within the forelimb and tendon lesions was assessed with scintigraphy for 24 h.
Lesion uptake was higher with i.l. injection than with RLP, but MSC persistence decreased similarly over time in all 3 techniques. Intra-arterial RLP resulted in a better distribution of MSCs and a higher uptake at the lesion site than i.v. RLP. Limbs perfused i.a. on Day 10 showed greater accumulation of MSCs in the lesion than limbs perfused on Day 3. Arterial thrombosis occurred in 50% of the i.v. RLP limbs and in 100% of the i.a. RLP limbs, which led to clinical complications in one horse.
Conclusions and potential relevance
Compared with i.l. injection, RLP results in lower uptake but similar persistence of MSCs at the site of tendon lesions. A time dependent accumulation of MSCs was identified with i.a. RLP. The i.a. RLP appears more advantageous than the i.v. RLP in terms of distribution and uptake. However, the described i.a. technique produced arterial thrombosis and thus cannot currently be recommended for clinical use.
The Summary is available in Chinese – see Supporting information.