EXPERIMENTAL AND BASIC RESEARCH STUDIES
Attempts to induce nocardioform placentitis (Crossiela equi) experimentally in mares
Article first published online: 1 APR 2014
© 2014 EVJ Ltd
Equine Veterinary Journal
Volume 47, Issue 1, pages 91–95, January 2015
How to Cite
Canisso, I. F., Ball, B. A., Erol, E., Claes, A., Scoggin, K. E., McDowell, K. J., Williams, N. M., Dorton, A. R., Wolfsdorf, K. E., Squires, E. L. and Troedsson, M. H. T. (2015), Attempts to induce nocardioform placentitis (Crossiela equi) experimentally in mares. Equine Veterinary Journal, 47: 91–95. doi: 10.1111/evj.12249
- Issue published online: 22 DEC 2014
- Article first published online: 1 APR 2014
- Accepted manuscript online: 24 FEB 2014 12:05PM EST
- Manuscript Accepted: 18 FEB 2014
- Manuscript Received: 23 AUG 2013
- Kentucky Thoroughbred Association/Kentucky Thoroughbred Breeders and Owners
- University of Kentucky (Department of Veterinary Science, Albert Clay Endowment and Geoffrey Hughes fellowship)
- premature parturition;
Reasons for performing study
Nocardioform placentitis in horses is poorly understood, and the development of an experimental model would be of help in understanding the pathogenesis of the disease.
To investigate whether (1) intrauterine inoculation of Crossiela equi during the periovulatory period or (2) i.v., oral or intranasopharyngeal inoculation of C. equi during midgestation would result in nocardioform placentitis, and (3) before and after mating endometrial swabs present evidence of nocardioform placentitis-associated organisms (C. equi or Amycolatopsis spp.).
In Study I, mares (n = 20) received an intrauterine inoculation of C. equi 24 h after artificial insemination. Endometrial swabs were obtained 24 h post inoculation for PCR analysis. In Study II, pregnant mares (at 180–240 days of gestation) were inoculated with C. equi by intranasopharyngeal (n = 5), oral (n = 4) or i.v. (n = 4) routes. Sixty contemporaneous pregnant mares maintained on the same farm served as control animals. In Study III, privately owned Thoroughbred mares (n = 200) had endometrial swabs collected before and within 24–48 h after mating for detection of nocardioform microorganisms.
In Study I, C.equi was identified by PCR in 3 of 20 mares following intrauterine inoculation. Pregnancy was established in 19 of 20 treated mares. There were 2 embryonic losses and one abortion at 177 days of gestation (undetermined cause). Sixteen mares delivered a normal foal and placenta. In Study II, one mare (oral inoculation) aborted at 200 days of gestation (unidentified cause). The remaining mares delivered a normal foal and placenta. In Study III, none of the mares yielded positive endometrial PCR for nocardioform microorganisms.
We were unable to induce nocardioform placentitis, and there was no evidence of nocardioform microorganisms in endometrial swabs of broodmares before or after mating. These findings suggest that nocardioform placentitis is not induced simply via the presence of nocardiform actinomycetes and that route, insufficient duration of exposure and dose may play a role in the development of disease. Additional predispositions may also be involved in the development of nocardioform placentitis.