Figure S1. Flow chart showing the number of colonies that were initially sampled (white boxes) and resampled (grey boxes) in each sampling period (1996, 2000-2002, 2005-2007, and 2009-2010, x-axis).

Figure S2. Map showing the distribution of colonies in our focal population along the Rhône River in Valais, Switzerland.

Figure S3. The distribution of intracolony relatedness estimates in 2009 to 2010.

Figure S4. Intracolony relatedness comparisons.

Table S1. Three-level hierarchical F-analysis of variance in which individuals were nested within colonies, colonies within patches, and patches within the full population, using the genotypes from 2009 to 2010 (N = 117 colonies).

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