STOCHASTIC TEMPERATURES IMPEDE RNA VIRUS ADAPTATION
Article first published online: 31 JAN 2013
© 2013 The Author(s). Evolution© 2013 The Society for the Study of Evolution.
Volume 67, Issue 4, pages 969–979, April 2013
How to Cite
Alto, B. W., Wasik, B. R., Morales, N. M. and Turner, P. E. (2013), STOCHASTIC TEMPERATURES IMPEDE RNA VIRUS ADAPTATION. Evolution, 67: 969–979. doi: 10.1111/evo.12034
- Issue published online: 3 APR 2013
- Article first published online: 31 JAN 2013
- Received July 28, 2012 Accepted November 8, 2012
- Adaptive constraints;
- ecological generalization and specialization;
- fitness consequences;
- RNA virus
Constant environments are often assumed to favor the evolution of specialization whereas exposure to changing environments may favor the evolution of generalists. Here we explored the phenotypic and molecular changes associated with evolving an RNA virus in constant versus fluctuating temperature environments. We used vesicular stomatitis virus (VSV) to determine whether selection at a constant temperature entails a performance trade-off at an unselected temperature, whether virus populations evolve to be generalists when selected in deterministically changing temperature environments, and whether selection under stochastically changing temperatures prevents evolved generalization, such as by constraining the ability for viruses to adaptively improve. We observed that all VSV lineages evolved at constant temperatures showed fitness gains in their selected temperature with little evidence for trade-offs in performance in the unselected environment. Evolution in deterministically and stochastically changing temperatures led to populations with the highest and lowest overall fitness gains, respectively. Sequence analysis revealed little evidence for convergent molecular evolution among lineages within the same treatment. Across all temperature treatments, the majority of genome substitutions occurred in the G (glycoprotein) gene, suggesting that this locus for the cell-binding protein plays a key role in dictating VSV performance under changing temperature.