Table S1. Fitness (ln (W)) of each 29°C- and 37°C-evolved VSV population.

Table S2. Fitness (ln (W)) of each evolved VSV population exposed to deterministic and stochastically changing temperature regimes compared to MARM C ancestor.

Table S3. Fitness (ln (W)) of H (high temperature) and L (low temperature) treatments after selection at constant 37°C and 29°C and assayed at 37°C and 29°C.

Table S4. Nonparametric tests for the number of new alleles across temperature treatments in vesicular stomatitis virus nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and large polymerase protein (L) genes.

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