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Table S1. Promiscuity and genetic diversity information for species used in intron analyses.

Table S2. Specific intron information (primer sequences, sequence lengths, and PCR details).

Table S3. Nucleotide diversity estimates (π) for the individual introns.

Table S4. Promiscuity measures and population origin for the eight species screened for MHC IIB exon 2 diversity.

Table S5. Sequence information for the eight species screened for MHC IIB exon 2 diversity.

Table S6. Principal component analysis of MHC IIB exon 2 diversity estimates.

Table S7. Species-specific diversity estimates for the complete MHC IIB exon 2, the peptide-binding sites (PBS) and the nonpeptide binding sites (non-PBS).

Table S8. Codon-based tests for positive selection on MHC IIB exon 2 in eight species.

Figure S1. Nucleotide diversity (π) of all introns, only autosomal introns and only Z-chromosome introns plotted against female promiscuity expressed as the coefficient of between-male variation in total sperm length (CVbm) for eight species.

Figure S2. NeighbourNet network illustrating sequence clusters in the MHC IIB alleles based on Kimura 2 parameter distances for eight species.

Figure S3. MHC IIB diversity (PC1) plotted against the coefficient of between-male variation in total sperm length (CVbm) for the complete exon 2, only the peptide-binding sites, and only the nonpeptide binding sites in eight study species.

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