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Figure S1. Phylogeny of ZF domain insulator proteins.

Figure S2. Phylogeny of BTB domain insulator proteins.

Figure S3. Absence of GAGA factor orthologs from vertebrates I.

Figure S4. Absence of GAGA factor orthologs from vertebrates II.

File S1. Multiple sequence alignment of ZF domain proteins.

File S2. Multiple sequence alignment of BTB domain proteins.

File S3. Multiple sequence alignment of vertebrate GAGA factor candidates (BLAST).

File S4. Multiple sequence alignment of vertebrate GAGA factor candidates (HMM).

Table S1. The top 40 arthropod species providing ZF candidate sequences.

Table S2. The top 40 arthropod species providing BTB candidate sequences.

Table S3. Download location of 26 genomes and proteomes used for a HMM/OrthoMCL-based search for insulator proteins.

Table S4. Summary of the HMM/OrthoMCL-based search for insulator proteins.

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