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Figure S1. Bayesian inference (BI) phylogram from act, cal ITS nrDNA, rpb2, and tef1 sequence data.

Figure S2. Distribution of P-values for likelihood ratio test (LRT) of BiSSE Ln Likelihood Difference inline image.

Figure S3. Summarized results of Branch-Site REL diversifying selection analysis.

Table S1. Taxa and strains used in the analyses with their corresponding GenBank accession numbers.

Table S2. Results of model estimation under Akaike Information Criterion (AIC; jMODELTEST) for each of the partitions.

Table S3. Taxa and character state coding.

Table S4. Results of parsimony ancestral character reconstruction for trait 1 (habitat preference), optimized using most parsimonious reconstruction (MPR).

Table S5. Summary of changes over trees in character (trait 1, habitat preference), for parsimony ancestral character reconstruction (ACR).

Table S6. Results of maximum likelihood ancestral character reconstruction for trait 1 (habitat preference).

Table S7. Results of parsimony ancestral character reconstruction for trait 2 (nutrition mode), a priori, optimized using most parsimonious reconstruction (MPR).

Table S8. Summary of changes over trees in character (trait 2, nutrition mode), a priori, for parsimony ancestral character reconstruction (ACR).

Table S9. Results of maximum likelihood ancestral character reconstruction for trait 2 (nutrition mode), a priori.

Table S10. Summarizing changes over trees in character (trait 2, nutrition mode), for likelihood ancestral character reconstruction (ACR), a priori.

Table S11. Results of Parsimony ancestral character reconstruction for trait 2 (nutrition mode), a posteriori, optimized using most parsimonious reconstruction (MPR).

Table S12. Summarizing changes over trees in character (trait 2, nutrition mode), a posteriori, for parsimony.

Table S13. Results of maximum likelihood ancestral character reconstruction for trait 2 (nutrition mode), a posteriori.

Table S14. Summarizing changes over trees in character (trait 2, nutrition mode), for likelihood ancestral character reconstruction (ACR), a posteriori.

Table S15. Results of Branch-Site REL analysis on the rpb2 data set.

Table S16. Results of Branch-Site REL analysis on the tef1 data set.

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