SEARCH

SEARCH BY CITATION

Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.

FilenameFormatSizeDescription
evo12183-sup-0001-supmat.pdf3688KFigure S1. Constitutive production of pyoverdin by mutant PBR852.
evo12183-sup-0002-supmat.pdf62KFigure S2. Growth curves of Pvd+ and pyoverdin constitutive (Pvdc) genotypes.
evo12183-sup-0003-supmat.pdf58KFigure S3. Population density of monocultures of Pvd+ and pyoverdin constitutive (Pvdc) genotypes in structured and unstructured microcosms.
evo12183-sup-0004-supmat.pdf89KFigure S4. Fitness of Pvd+ (ancestral) and Pvd (PBR851 and PBR840) genotypes in structured and unstructured microcosms at different initial founding frequencies.
evo12183-sup-0005-supmat.pdf2571KFigure S5. A proposed model for the evolution of Pvd mutants in structured microcosms based on current understanding of pyoverdin regulation in Pseudomonas fluorescens SBW25.
evo12183-sup-0006-supmat.pdf92K

Table S1. Bacterial strains, plasmids, and oligonucleotide primers used in this study.

Table S2. Frequency of Pvd- mutants (percentage) and production of pyoverdin in the structured and unstructured microcosms after ∼600 generations of evolution.

Table S3. Summary analysis of variance results: genotype (PBR851 vs. PBR840) effect in King's Medium B.

Table S4. Summary analysis of variance results: PBR851 in King's Medium B.

Table S5. Summary analysis of variance results: PBR840 in King's Medium B.

Table S6. Summary analysis of variance results: genotype (PBR851 vs. PBR840) effect in casamino acids medium.

Table S7. Summary analysis of variance results: PBR851 in casamino acids medium.

Table S8. Summary analysis of variance results: PBR840 in casamino acids medium.

Table S9. Summary analysis of variance results: medium (King's Medium B vs. casamino acids medium) effect.

evo12183-sup-0007-supmat.jmp62KTable S10. Raw data for fitness assays in excel format.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.