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Table S1. Principal component analysis loadings for male and female behavior.

Table S2. Zero-spot trainer females were more active than two-spot trainer females.

Table S3. Male DHSN did not influence trainer male behavior.

Table S4. Sex did not significantly influence trainer behavior.

Table S5. Female activity levels did not significantly influence male preference learning.

Table S6. Power analysis to calculate effect sizes for detecting statistically significant effect of premating experience.

Figure S1. Wing manipulations performed in this study.

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