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Figure S1. The mean locomotor activity TPCs for (a) females and (b) males of each mutation accumulation line.

Figure S2. Reanalysis of data in Figure 3 of the main text including only those MA lines that showed a unimodal activity curve (males 24 lines, females 16 lines).

Table S1. Univariate mixed effects linear models testing for the effects of mating treatment, block, line, and vial on locomotor activity at each of the six test temperatures in male and female Drosophila serrata.

Table S2. Eigenvalues and vectors of the mutational variance–covariance matrices, M, for locomotor activity across six temperatures for Drosophila serrata.

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