Original Article

Why in vivo may not equal in vitro – new effectors revealed by measurement of enzymatic activities under the same in vivo-like assay conditions

  1. Rodolfo García-Contreras1,2,
  2. Paul Vos3,
  3. Hans V. Westerhoff1,4,5,
  4. Fred C. Boogerd1,*

Article first published online: 12 OCT 2012

DOI: 10.1111/febs.12007

Issue

FEBS Journal

FEBS Journal

Volume 279, Issue 22, pages 4145–4159, November 2012

Additional Information

How to Cite

García-Contreras, R., Vos, P., Westerhoff, H. V. and Boogerd, F. C. (2012), Why in vivo may not equal in vitro – new effectors revealed by measurement of enzymatic activities under the same in vivo-like assay conditions. FEBS Journal, 279: 4145–4159. doi: 10.1111/febs.12007

Author Information

  1. 1

    Section of Molecular Cell Physiology, Netherlands Institute for Systems Biology, VU University Amsterdam, The Netherlands

  2. 2

    Departamento de Bioquímica, Instituto Nacional de Cardiología, Mexico City, México

  3. 3

    Department of Methodology and Applied Biostatistics, VU University Amsterdam, The Netherlands

  4. 4

    Manchester Centre for Integrative Systems Biology, The University of Manchester, UK

  5. 5

    Synthetic Systems Biology, University of Amsterdam, The Netherlands

* Correspondence
F. C. Boogerd, Section of Molecular Cell Physiology, Netherlands Institute for Systems Biology, VU University Amsterdam, The Netherlands
Fax: +31 20 5987229
Tel.: +31 20 5987194
E-mail: fred.boogerd@falw.vu.nl
Website: http://www.bio.vu.nl/vakgroepen/mcp

Publication History

  1. Issue published online: 29 OCT 2012
  2. Article first published online: 12 OCT 2012
  3. Accepted manuscript online: 14 SEP 2012 07:57AM EST
  4. Manuscript Accepted: 10 SEP 2012
  5. Manuscript Revised: 9 AUG 2012
  6. Manuscript Received: 10 JUL 2012

Funded by

  • FP6-NEST-STREP project. Grant Number: 043235
  • BBSRC, EPSRC. Grant Numbers: BBD0190791, BBC0082191, BBF0035281, BBF0035521, BBF0035521, BBF0035361, BBG5302251, SySMO P49
  • EU-FP7
  • BioSim, NucSys
  • UniCellSys and ZON-MW. Grant Number: 91206069

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