These authors contributed equally to this work
A diverse set of family 48 bacterial glycoside hydrolase cellulases created by structure-guided recombination
Version of Record online: 9 NOV 2012
© 2012 The Authors Journal compilation © 2012 FEBS
The FEBS Journal
Volume 279, Issue 24, pages 4453–4465, December 2012
How to Cite
Smith, M. A., Rentmeister, A., Snow, C. D., Wu, T., Farrow, M. F., Mingardon, F. and Arnold, F. H. (2012), A diverse set of family 48 bacterial glycoside hydrolase cellulases created by structure-guided recombination. The FEBS Journal, 279: 4453–4465. doi: 10.1111/febs.12032
- Issue online: 4 DEC 2012
- Version of Record online: 9 NOV 2012
- Accepted manuscript online: 17 OCT 2012 12:00AM EST
- Manuscript Accepted: 12 OCT 2012
- Manuscript Revised: 10 OCT 2012
- Manuscript Received: 16 JUN 2012
- Defense Advanced Research Projects Agency
- Resnick Sustainability Institute
Doc. S1. CelY synthetic complete gene sequence. Fig. S1. Catalytic domains of CelY, CelF and CelS and their sequence identities.
Fig. S2. CelS Xba1 site and CelY DUF do not affect cellulolytic activities.
Fig. S3. SCHEMA library designs with eight blocks and a minimum block length of 30 amino acids.
Fig. S4. Naming scheme for primers used for SCHEMA library construction.
Fig. S5. Comparison of T50 thermostability models for the three parents and 50 chimeras.
Fig. S6. CD measurements of several functional and nonfunctional chimeras.
Fig. S7. Examples of T50 measurements, with incubation in the absence and presence of miniscaffoldin and substrate.
Fig. S8. High-throughput screen for activity of Cel48 chimeras on crystalline cellulose.
Table S1. Library blocks of the Cel48 SCHEMA library.
Table S2. Coefficients of the functionality model.
Table S3. The functional model.
Table S4. Cellulolomal chimeras constructed, along with the predicted functionality and T50s of the chimeras and measured functionality and T50s.
Table S5. Primer names and primer sequences used for parent constructs.
Table S6. List of primers used for construction of the SCHEMA library.
Table S7. The quality of the built library.
Equation S1. The thermostability model.
Equation S2. The functionality model.
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