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Fig. S1. Stable products of Hs-RNase H1 cleavage of the 21-bp RNA·DNA heteroduplex substrate.

Fig. S2. Comparison of heteroduplex substrate cleavage by Hs-RNase H1, with or without an N-terminal (His)6-tag.

Fig. S3. Comparison of the Mg2+ titration behavior of Hs-RNase H1 and the H264A mutant.

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