Evidence for a dual functional role of a conserved histidine in RNA·DNA heteroduplex cleavage by human RNase H1
Article first published online: 9 NOV 2012
© 2012 The Authors Journal compilation © 2012 FEBS
Volume 279, Issue 24, pages 4492–4500, December 2012
How to Cite
Alla, N. R. and Nicholson, A. W. (2012), Evidence for a dual functional role of a conserved histidine in RNA·DNA heteroduplex cleavage by human RNase H1. FEBS Journal, 279: 4492–4500. doi: 10.1111/febs.12035
- Issue published online: 4 DEC 2012
- Article first published online: 9 NOV 2012
- Accepted manuscript online: 18 OCT 2012 12:00AM EST
- Manuscript Accepted: 15 OCT 2012
- Manuscript Revised: 12 OCT 2012
- Manuscript Received: 25 JUN 2012
Fig. S1. Stable products of Hs-RNase H1 cleavage of the 21-bp RNA·DNA heteroduplex substrate.
Fig. S2. Comparison of heteroduplex substrate cleavage by Hs-RNase H1, with or without an N-terminal (His)6-tag.
Fig. S3. Comparison of the Mg2+ titration behavior of Hs-RNase H1 and the H264A mutant.
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