M. Wu and W. Nerinckx contributed equally to this study
Rational design, synthesis, evaluation and enzyme–substrate structures of improved fluorogenic substrates for family 6 glycoside hydrolases
Article first published online: 7 DEC 2012
© 2012 The Authors Journal compilation © 2012 FEBS
Volume 280, Issue 1, pages 184–198, January 2013
How to Cite
Wu, M., Nerinckx, W., Piens, K., Ishida, T., Hansson, H., Sandgren, M. and Ståhlberg, J. (2013), Rational design, synthesis, evaluation and enzyme–substrate structures of improved fluorogenic substrates for family 6 glycoside hydrolases. FEBS Journal, 280: 184–198. doi: 10.1111/febs.12060
- Issue published online: 3 JAN 2013
- Article first published online: 7 DEC 2012
- Accepted manuscript online: 9 NOV 2012 06:56AM EST
- Manuscript Accepted: 5 NOV 2012
- Manuscript Revised: 31 OCT 2012
- Manuscript Received: 31 AUG 2012
- Faculty for Natural Resources and Agriculture, Swedish University of Agricultural Sciences; Swedish Farmers Research Foundation (SLF); Swedish Energy Agency
- MicroDrivE – Microbially Derived Energy
- Japan Society for the Promotion of Science (JSPS)
Fig. S1. Example of fluorescence emission wavelength spectra showing that the fluorescence of the fluorogenic substrate is negligible compared to the corresponding free fluorophore.
Fig. S2. The productive poses of the umbelliferyl-β–cellobioside substrates obtained in the ligand docking, superposed with MUF–G2 and ClPhUF–G2 bound at the catalytic centre in the H. jecorina D221A complex structures.
Table S1. Summary of statistics from X-ray data collection, processing and refinement of the H. jecorina D221A complex structures.
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