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Fig. S1. Example of fluorescence emission wavelength spectra showing that the fluorescence of the fluorogenic substrate is negligible compared to the corresponding free fluorophore.

Fig. S2. The productive poses of the umbelliferyl-β–cellobioside substrates obtained in the ligand docking, superposed with MUF–G2 and ClPhUF–G2 bound at the catalytic centre in the H. jecorina D221A complex structures.

Table S1. Summary of statistics from X-ray data collection, processing and refinement of the H. jecorina D221A complex structures.

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