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Fig. S1. Alignment of the deduced amino acid sequence of DtGUP1 and other MBOAT family proteins.

Fig. S2. The transmembrane region prediction was performed at http://www.ch.embnet.org/.

Fig. S3. Generation of the transformant DtGUP1-E.

Fig. S4. Microscopic observation of Dunaliella tertiolecta wild-type and mutant DtGUP1-E during hyperosmotic shock.

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