• 1
    Tumpey TM & Belser JA (2009) Resurrected pandemic influenza viruses. Annu Rev Microbiol 63, 7998.
  • 2
    Stamboulian D, Bonvehi PE, Nacinovich FM & Cox N (2000) Influenza. Infect Dis Clin North Am 14, 141166.
  • 3
    Hampson AW & Mackenzie JS (2006) The influenza viruses. Med J Aust 185, S39S43.
  • 4
    Du X, Dong L, Lan Y, Peng Y, Wu A, Zhang Y, Huang W, Wang D, Wang M, Guo Y et al. (2012) Mapping of H3N2 influenza antigenic evolution in China reveals a strategy for vaccine strain recommendation. Nat Commun 3, 709.
  • 5
    de Jong JC, Beyer WE, Palache AM, Rimmelzwaan GF & Osterhaus AD (2000) Mismatch between the 1997/1998 influenza vaccine and the major epidemic A(H3N2) virus strain as the cause of an inadequate vaccine-induced antibody response to this strain in the elderly. J Med Virol 61, 9499.
  • 6
    Nelson MI & Holmes EC (2007) The evolution of epidemic influenza. Nat Rev Genet 8, 196205.
  • 7
    Arino J, Bowman CS & Moghadas SM (2009) Antiviral resistance during pandemic influenza: implications for stockpiling and drug use. BMC Infect Dis 9, 8.
  • 8
    Colman PM (2009) New antivirals and drug resistance. Annu Rev Biochem 78, 95118.
  • 9
    Boltz DA, Aldridge JR Jr, Webster RG & Govorkova EA (2010) Drugs in development for influenza. Drugs 70, 13491362.
  • 10
    Salomon R & Webster RG (2009) The influenza virus enigma. Cell 136, 402410.
  • 11
    Hulse-Post DJ, Franks J, Boyd K, Salomon R, Hoffmann E, Yen HL, Webby RJ, Walker D, Nguyen TD & Webster RG (2007) Molecular changes in the polymerase genes (PA and PB1) associated with high pathogenicity of H5N1 influenza virus in mallard ducks. J Virol 81, 85158524.
  • 12
    Zhou B, Li Y, Halpin R, Hine E, Spiro DJ & Wentworth DE (2011) PB2 residue 158 is a pathogenic determinant of pandemic H1N1 and H5 influenza a viruses in mice. J Virol 85, 357365.
  • 13
    Salomon R, Franks J, Govorkova EA, Ilyushina NA, Yen HL, Hulse-Post DJ, Humberd J, Trichet M, Rehg JE, Webby RJ et al. (2006) The polymerase complex genes contribute to the high virulence of the human H5N1 influenza virus isolate A/Vietnam/1203/04. J Exp Med 203, 689697.
  • 14
    Ruigrok RW, Crepin T, Hart DJ & Cusack S (2010) Towards an atomic resolution understanding of the influenza virus replication machinery. Curr Opin Struct Biol 20, 104113.
  • 15
    Wunderlich K, Mayer D, Ranadheera C, Holler AS, Manz B, Martin A, Chase G, Tegge W, Frank R, Kessler U et al. (2009) Identification of a PA-binding peptide with inhibitory activity against influenza A and B virus replication. PLoS One 4, e7517.
  • 16
    Su CY, Cheng TJ, Lin MI, Wang SY, Huang WI, Lin-Chu SY, Chen YH, Wu CY, Lai MM, Cheng WC et al. (2010) High-throughput identification of compounds targeting influenza RNA-dependent RNA polymerase activity. Proc Natl Acad Sci USA 107, 1915119156.
  • 17
    Reuther P, Manz B, Brunotte L, Schwemmle M & Wunderlich K (2011) Targeting of the influenza A virus polymerase PB1-PB2 interface indicates strain-specific assembly differences. J Virol 85, 1329813309.
  • 18
    Deng T, Sharps J, Fodor E & Brownlee GG (2005) In vitro assembly of PB2 with a PB1-PA dimer supports a new model of assembly of influenza A virus polymerase subunits into a functional trimeric complex. J Virol 79, 86698674.
  • 19
    Huet S, Avilov SV, Ferbitz L, Daigle N, Cusack S & Ellenberg J (2010) Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy. J Virol 84, 12541264.
  • 20
    Manz B, Gotz V, Wunderlich K, Eisel J, Kirchmair J, Stech J, Stech O, Chase G, Frank R & Schwemmle M (2011) Disruption of the viral polymerase complex assembly as a novel approach to attenuate influenza A virus. J Biol Chem 286, 84148424.
  • 21
    Ohtsu Y, Honda Y, Sakata Y, Kato H & Toyoda T (2002) Fine mapping of the subunit binding sites of influenza virus RNA polymerase. Microbiol Immunol 46, 167175.
  • 22
    Poole EL, Medcalf L, Elton D & Digard P (2007) Evidence that the C-terminal PB2-binding region of the influenza A virus PB1 protein is a discrete alpha-helical domain. FEBS Lett 581, 53005306.
  • 23
    He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A, Zeng Z et al. (2008) Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature 454, 11231126.
  • 24
    Sugiyama K, Obayashi E, Kawaguchi A, Suzuki Y, Tame JR, Nagata K & Park SY (2009) Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase. EMBO J 28, 18031811.
  • 25
    Chase G, Wunderlich K, Reuther P & Schwemmle M (2011) Identification of influenza virus inhibitors which disrupt of viral polymerase protein-protein interactions. Methods 55, 188191.
  • 26
    Ghanem A, Mayer D, Chase G, Tegge W, Frank R, Kochs G, Garcia-Sastre A & Schwemmle M (2007) Peptide-mediated interference with influenza A virus polymerase. J Virol 81, 78017804.
  • 27
    Morell M, Czihal P, Hoffmann R, Otvos L, Aviles FX & Ventura S (2008) Monitoring the interference of protein-protein interactions in vivo by bimolecular fluorescence complementation: the DnaK case. Proteomics 8, 34333442.
  • 28
    Hu CD, Chinenov Y & Kerppola TK (2002) Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9, 789798.
  • 29
    Wang JG, Chen CH, Chien CT & Hsieh HL (2011) FAR-RED INSENSITIVE219 modulates CONSTITUTIVE PHOTOMORPHOGENIC1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiol 156, 631646.
  • 30
    Deng Q, Zhai JW, Michel ML, Zhang J, Qin J, Kong YY, Zhang XX, Budkowska A, Tiollais P, Wang Y et al. (2007) Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site. J Virol 81, 42444254.
  • 31
    Hammarstrom M, Hellgren N, van Den Berg S, Berglund H & Hard T (2002) Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli. Protein Sci 11, 313321.
  • 32
    Waugh DS (2005) Making the most of affinity tags. Trends Biotechnol 23, 316320.
  • 33
    Zhu W, Zhou J, Qin K, Du N, Liu L, Yu Z, Zhu Y, Tian W, Wu X & Shu Y (2011) A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity. Virol J 8, 29.
  • 34
    Obayashi E, Yoshida H, Kawai F, Shibayama N, Kawaguchi A, Nagata K, Tame JR & Park SY (2008) The structural basis for an essential subunit interaction in influenza virus RNA polymerase. Nature 454, 11271131.
  • 35
    Emerson SD, Palermo R, Liu CM, Tilley JW, Chen L, Danho W, Madison VS, Greeley DN, Ju G & Fry DC (2003) NMR characterization of interleukin-2 in complexes with the IL-2Ralpha receptor component, and with low molecular weight compounds that inhibit the IL-2/IL-Ralpha interaction. Protein Sci 12, 811822.
  • 36
    Wilson CG & Arkin MR (2011) Small-molecule inhibitors of IL-2/IL-2R: lessons learned and applied. Curr Top Microbiol Immunol 348, 2559.
  • 37
    Schneidman-Duhovny D, Inbar Y, Nussinov R & Wolfson HJ (2005) PatchDock and SymmDock: servers for rigid and symmetric docking. Nucleic Acids Res 33, W363W367.
  • 38
    Zhang H, Chen J, Wang Y, Peng L, Dong X, Lu Y, Keating AE & Jiang T (2009) A computationally guided protein-interaction screen uncovers coiled-coil interactions involved in vesicular trafficking. J Mol Biol 392, 228241.
  • 39
    Wang Y, Zhang X, Zhang H, Lu Y, Huang H, Dong X, Chen J, Dong J, Yang X, Hang H et al. (2012) Coiled-coil networking shapes cell molecular machinery. Mol Biol Cell 23, 39113922.
  • 40
    Melcher K (2000) A modular set of prokaryotic and eukaryotic expression vectors. Anal Biochem 277, 109120.
  • 41
    Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG & Garcia-Sastre A (1999) Rescue of influenza A virus from recombinant DNA. J Virol 73, 96799682.
  • 42
    Ohad N, Shichrur K & Yalovsky S (2007) The analysis of protein-protein interactions in plants by bimolecular fluorescence complementation. Plant Physiol 145, 10901099.