Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides
Version of Record online: 21 FEB 2013
© 2013 The Authors Journal compilation © 2013 FEBS
The FEBS Journal
Volume 280, Issue 6, pages 1475–1490, March 2013
How to Cite
Zhou, X., Szeker, K., Janocha, B., Böhme, T., Albrecht, D., Mikhailopulo, I. A. and Neubauer, P. (2013), Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides. The FEBS Journal, 280: 1475–1490. doi: 10.1111/febs.12143
- Issue online: 12 MAR 2013
- Version of Record online: 21 FEB 2013
- Accepted manuscript online: 19 JAN 2013 08:15AM EST
- Manuscript Accepted: 16 JAN 2013
- Manuscript Revised: 13 JAN 2013
- Manuscript Received: 6 DEC 2012
- Deutsche Forschungsgemeinschaft (DFG). Grant Number: EXC 314
Fig. S1. Relative pH-dependent activity of the thermostalbe PNPs with natural and modified substrates.
Fig. S2. Spectral chromatograms for synthesized dAdo2′F and dAdo2′F.
Table S1. Identification of ApMTAP by MALDI-TOF MS quatered measurements.
Table S2. Peptides identification of ApMTAP by MALDI-TOF MS and MS/MS.
Table S3. pH-dependent activity of the thermostalbe PNPs with natural and modified substrates.
Table S4. The structural and electronic features of Ado, dAdo2′NH2, dAdo2′F and dAdo2′F.
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