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Fig. S1. Relative pH-dependent activity of the thermostalbe PNPs with natural and modified substrates.

Fig. S2. Spectral chromatograms for synthesized dAdo2′F and dAdo2′F.

Table S1. Identification of ApMTAP by MALDI-TOF MS quatered measurements.

Table S2. Peptides identification of ApMTAP by MALDI-TOF MS and MS/MS.

Table S3. pH-dependent activity of the thermostalbe PNPs with natural and modified substrates.

Table S4. The structural and electronic features of Ado, dAdo2′NH2, dAdo2′F and dAdo2′F.

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